Abstract
The glucoamylase 1 gene (PnGlu1) from the saprophytic fungus Pholiota microspora has been previously cloned and characterized. In this study, a second glucoamylase gene (PnGlu2), 1719 bp long and encoding 573 amino acids, was identified in the P. microspora genome. PnGlu1 and PnGlu2 show 70% amino acid identity. Transcriptional analyses showed that PnGlu1 and PnGlu2 expression in minimal media containing different carbon sources are far lower than that in sawdust medium. In sawdust medium, transcription level of PnGlu1 is high in vegetative dikaryotic mycelia and that of PnGlu2 is high in primordia and at the fruiting body stage. These data suggested that the two glucoamylase gene PnGlu1 and PnGlu2 may be differentially regulated in each developmental stage such as mycelial growth and fruiting body formation.
