Abstract
The protective effect of volatile compounds from spent substrates of shiitake (Lentinula edodes) and bunashimeji (Hypsizygus marmoreus) against Alternaria sooty spot of cabbage was investigated. Cabbage plants exposed to volatile compounds from both spent substrates were protected significantly from formation of necrotic spots on the leaves by Alternaria brassicicola (O-264 isolate). Because spent substrate of shiitake had a protective effect at a lower amount than bunashimeji, volatiles from the spent substrate of shiitake were tested for antifungal activity against O-264 spores. The germ tube elongation of spores was inhibited by spent substrate of shiitake, but the inhibition disappeared when the spent substrate was removed, suggesting the presence of fungistatic volatiles. Then, volatiles from the spent substrate of shiitake were tested for the induction of systemic disease resistances. Cabbage plants treated with the volatiles were protected from necrotic spots caused by O-264 even after removal of the spent substrate, implying that the volatiles from spent substrate of shiitake induced systemic disease resistance.
