Abstract
To develop artificial cultivation of Tricholoma matsutake, it is necessary to establish stable culture conditions under which mycelia can spread quickly. However, an advantageous solid-state culture method for this fungus has not yet been identified. We developed a solid-state culture medium using barley and vermiculite to obtain a large amount of T. matsutake mycelia in a short time, and we estimated the T. matsutake fungal biomass in this artificial medium. We determined optimal conditions for mycelial biomass quantification through measurement of n-acetylglucosamine (GlcNAc) concentration in the mycelia. For degrading dry T. matsutake mycelia, 1.0% Yatalase and 0.5% Cellulase “ONOZUKA” RS solution provided optimal degradation conditions, and 139 μg GlcNAc per 10 mg of dried mycelia was produced. Subsequently, T. matsutake Z-1, NBRC 30605, and strain No. 115 were tested and demonstrated good growth using medium with barley:vermiculite composition of 2:1 (w/w). After 35 days of cultivation, T. matsutake Z-1, NBRC 30605, and strain No. 115 produced 215.1, 254.0, and 266.7 mg biomass/flask, respectively. By both visual observation and measurement of GlcNAc content in colonized substrate block, a 2:1 barley:vermiculite composition was demonstrated to be the optimum medium for the culture of T. matsutake mycelia.
