Screening of non-fruiting and fruiting monokaryons from basidiospore isolates from Pleurotus salmoneostramineus NBRC31859

Abstract

Establishing a model strain can be an effective means to research the fruiting body formation of mushrooms using genetic approaches. Pleurotus salmoneostramineus L. Vass is known for its pink color. The mushroom is easy to cultivate in the laboratory, and it develops a clear pink color when the primordia are formed. By morphological observation of hyphae and fruiting bodies, and the compatibility test, it was revealed that it is a quadrupolar mushroom with a heterothallic lifecycle. Here, we screened monosporous strains isolated from P. salmoneostramineus NBRC31859. Among these, 30 strains showed a variety of mycelial and fruiting types, and monokaryotic strains N81 (non-fruiting) and N13 (fruiting) were selected as model strains for fruiting mechanism experiments based on their similar mycelial growth rate and colony morphology.