2021 Volume 28 Issue 4 Pages 182-186
Ezoharitake (Climacodon septentrionalis) is an edible mushroom in the Phanerochaetaceae family. Biological activities and novel compounds in mycelial and fruiting body extracts have been reported and they could be useful bioresources. However, the effective cultivation methods of this mushroom have not been sufficiently studied. In this study, when mycelial running was completed, the culture medium was subjected to flushing treatments that included scratching, thumping, casing, and bag-cutting. A low yield of fruiting bodies was obtained in the medium treated with scratching and thumping. No fruiting body formation was observed in the medium treated with casing. A high yield of normal fruiting bodies was observed in the medium treated with bag-cutting. Short-term fruiting after bag cutting was observed in the medium cultured for 50 to 60 days at 25°C. Fruiting body formation was observed at 17°C and 19°C, but not at 15°C. The yield of fruiting bodies at 19°C was higher than that at 17°C. These results suggest that sustainable fruiting body formation can be achieved in medium cultured for 50 to 60 days, with bag-cutting treatment, and incubation at 19°C.
