Antioxidant activity of different parts of Grifola frondosa and fractionation of iron-binding proteins

Abstract

In this study, different parts of <i>Grifola frondosa</i> were analyzed to examine their antioxidant activity and transition metal content. For the examination, the fruiting body was divided into three parts and the medium into two parts. The cap showed the highest antioxidant activity. We found differences in the amounts of transition metals among different parts of the fruiting body. To purify the iron-binding proteins having antioxidant activity, we subjected the cap of G. frondosa to size-separation gel-filtration chromatography. A protein fraction was detected from the obtained chromatogram. This fraction was speculated to represent the iron-binding proteins.