Abstract
Laccaria bicolor was reported to harbor endosymbiont bacteria in hyphae. Detailed information of other Laccaria species has not been available. This study aims to identify and investigate endo-hyphal or endosymbiont bacteria not only from L. bicolor, but also from L. amethystina, and L. vinaceoavellanea. We prepared three types of strains, viz. Hyphae1 (original hyphae), Hyphae2 (eliminated exo-hyphal bacteria through agar filter), and Hyphae3 (treated with antibiotics). When we performed SYTO staining on Hyphae1 strain, many green spots were detected. Hyphae2 strain showed similar yet fewer green spots as those observed in Hyphae1. Hyphae3 strain showed red spots but no green spots. From Hyphae2, we isolated five endo-hyphal bacteria, MBLb1a, MBLa2a, MBLa2b, MBLv13a and MBLv13b. Molecular identification revealed that MBLb1a, MBLa2a and MBLa2b were Paenibacillus chitinolyticus, MBLv13a was Dietzia timorensis and MBLv13b was Kytococcus sedentarius. The co-culture study showed that significant stimulation was detected in the two pairings of L. vinaceoavellanea with Dietzia timorensis and with K. sedentarius on two kinds of media, 1/5 diluted MMN agar minus (NH4)2HPO4 and 1/5 diluted MMN agar minus KH2PO4.
