Abstract
Regarding the culture conditions for the mycelia of Chlorophyllum molybdites (Iwade strain 51), it was clarified that the optimal culture temperature for the mycelia is 25˚C, and the culture medium with rice bran added to bark compost ［bark compost: rice bran = 10 : 1 (w/w), moisture content 65% (wet basis)］ and the culture medium with corn steep liquor added to bark compost ［bark compost: corn steep liquor = 10 : 1 (w/w), moisture content 65% (wet basis)］ produce the fastest mycelial spreading. Regarding the cultivation conditions, it was confirmed that fruiting bodies were generated on the mushroom bed of bark compost with rice bran added ［bark compost: rice bran = 10 : 1 (w/w), moisture content 60 - 65% (wet basis)］, covered with black soil whose pH was adjusted to approximately 7.0 using oyster shell lime, by scratching the mycelia and sprinkling water.
https://ror.org/01w6wtk13 
