アフラトキシンB1 誘導ラット肝癌由来K2 細胞は癌幹細胞の性質を有している

川崎 靖; 安達 尚美; 山崎 富生; 轟 理沙; 後藤 良隆; 古宮 裕子; 倉部 誠也; 根本 清光; 出川 雅邦; 三浦 成敏; 田代 文夫

doi:10.2520/myco.57.87

Regular Papers

Cancer stem cells in aflatoxin B₁-induced rat hepatocellular carcinoma K2 cells

Yasushi KAWASAKI, Naomi ADACHI, Tomio YAMAZAKI, Risa TODOROKI, Yoshitaka GOTOU, Yuko KOMIYA, Nobuya KURABE, Kiyomitsu NEMOTO, Masakuni DEGAWA, Shigetoshi MIURA, Fumio TASHIRO

Author information

Yasushi KAWASAKI
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Naomi ADACHI
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Tomio YAMAZAKI
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Risa TODOROKI
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Yoshitaka GOTOU
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Yuko KOMIYA
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Nobuya KURABE
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Kiyomitsu NEMOTO
Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka
Masakuni DEGAWA
Department of Molecular Toxicology, School of Pharmaceutical Sciences, University of Shizuoka
Shigetoshi MIURA
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science
Fumio TASHIRO
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science

Keywords: afratoxin B₁, hepatocarcinogenesis, cancer stem cells, bmi1, side population

JOURNAL FREE ACCESS

2007 Volume 57 Issue 2 Pages 87-93

DOI https://doi.org/10.2520/myco.57.87

Details

Abstract

In this study, we performed the side population (SP) cell analysis and the expression analysis of stemness genes to identify stem-like cells (cancer stem cells) in aflatoxin B₁-(AFB₁) induced rat hepatoma K2 cells. Using the flow cytometric analysis with rhodamine 123, the SP cells, which are thought to be cancer stem cells, were detected with high frequency and estimated to be approximately 89 % of K2 cell population. Furthermore, K2 cells expressed stemness genes including the sox2, nanog, klf4, the ras family E-ras and the polycomb family bmi1 genes, which are implicated in the maintenance of pluripotency and self-renewal of embryonic stem (ES) cells. On the other hand, in K2 cells the expression levels of mature hepatocyte-specific marker genes such as albumin and tyrosine aminotransferase (TAT) were very low. Thus, these results imply that K2 cells possess the typical properties of cancer stem cells through the acquisition of stemness gene expressions.

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