To validate commercial kit for screening of the presence of AFB
1 in corn, an interlaboratory study was conducted to evaluate three quantitative and two qualitative immunoassay kits designed for the detection of aflatoxins. Four laboratories performed a screen for the presence of aflatoxin B
1 (AFB
1) in corn. As for the quantitative kits, repeatability relative standard deviation (RSD
r) and reproducibility relative standard deviation (RSD
R) were estimated. One laboratory evaluated the lot variation of each quantitative kit. All kits used in this study showed that the RSD
r and RSD
R were less than 23.3 % and 35.7 %, respectively, in spiked or naturally contaminated corn samples. As for the qualitative kits, neither false positive nor false negative results were found in corn samples (blank, spiked or naturally contaminated samples) in any laboratories. The RSDs in the lot variation of the same quantitative kit was less than 46.5 % in both two brands. The results appeared to indicate that all kits tested in this study could be validated for the screening of the presence of AFB
1 in corn, and were also available for the first step of the detection of AFB
1 at levels of more than 5 ng/g.
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