JSM Mycotoxins
Online ISSN : 1881-0128
Print ISSN : 0285-1466
ISSN-L : 0285-1466
Regular Papers
Production of 3-acetylnivalenol by transgenic Fusarium graminearum expressing Tri13 of type A trichothecene-producer: participation of the encoded cytochrome P450 monooxygenase in type B trichothecene biosynthesis
Kazuyuki MAEDATakeshi TOKAIHinayo ICHIKAWANaoko TAKAHASHI-ANDONobuo OGURAKatsuyoshi YONEYAMAMinoru YOSHIDAMakoto KIMURA
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2012 Volume 62 Issue 2 Pages 83-90

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Abstract

  Tri13 encodes a cytochrome P450 monooxygenase that catalyzes C-4 hydroxylation of some intermediates in the biosynthesis of type A and type B trichothecenes. This work demonstrated that Tri13 (FsTri13) of Fusarium sporotrichioides, a typical type A trichothecene producer, can participate in the biosynthesis of type B trichothecenes. When FsTri13 was expressed in Fusarium graminearum that produces 3-acetyldeoxynivalenol (3-ADON) as an end product, 3-acetylnivalenol (3-ANIV) was newly detected in the liquid medium. Although 3-ANIV can easily be prepared enzymatically from nivalenol (NIV) by using recombinant trichothecene 3-O-acetyltransferase (TRI101), there has been no conclusive report on the production of 3-ANIV in cultures of Fusarium strains. Possible biosynthetic pathway for production of 3-ANIV was suggested with regard to the function of Tri8, which is responsible for deacetylation of the side-chain O-acetyls in 3-ADON biosynthesis.

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© 2012 by Japanese Society of Mycotoxicology
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