Abstract
The contamination of milk with aflatoxin M1 (AFM1), a possible carcinogen to humans, is a serious problem. In Thailand, there are few reports on AFM1 contamination in powdered milk. In this study, we investigated the occurrence of AFM1 in 79 commercial powdered milk products in Bangkok, Thailand over five years (2010–2014). An in-house immunoaffinity column-HPLC (IAC-HPLC) method was developed. Reconstituted powdered milk (10 mL) was applied to our IAC. The recoveries from 0.005–0.15 ng/mL AFM1-spiked reconstituted milk were 73.6–86.3% with 5.2–7.4% of RSD. Twelve samples (15%) were contaminated with 0.005–0.135 ng/mL of AFM1 (average 0.024 ng/mL, overall average 0.004 ng/mL) in reconstituted powdered milk. Our data indicated that the risk of AFM1 in commercial powdered milk in Bangkok, Thailand was sufficiently low.
