デオキシニバレノールケモタイプのFusarium graminearumが生産するB型トリコテセン量の測定：HPLC-UV法とLC-MS法の比較

田中 彰; 新海 航輝; 前田 一行; 中嶋 佑一; 石井 茂; 吉田 泰彦; 木村 真; 安藤 直子

doi:10.2520/myco.69-1-2

Letter

Comparison of HPLC-UV and LC-MS methods for evaluating the amount of deoxynivalenol-type trichothecenes in axenic solid culture of Fusarium graminearum

Akira Tanaka, Koki Shinkai, Kazuyuki Maeda, Yuichi Nakajima, Shigeru Ishii, Yasuhiko Yoshida, Makoto Kimura, Naoko Takahashi-Ando

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Akira Tanaka
Graduate School of Engineering, Toyo University
Koki Shinkai
Graduate School of Science and Engineering, Toyo University
Kazuyuki Maeda
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Yuichi Nakajima
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Shigeru Ishii
Department of Science and Engineering, Toyo University
Yasuhiko Yoshida
Graduate School of Engineering, Toyo University
Graduate School of Science and Engineering, Toyo University
Department of Science and Engineering, Toyo University
Makoto Kimura
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Naoko Takahashi-Ando
Graduate School of Engineering, Toyo University
Graduate School of Science and Engineering, Toyo University
Department of Science and Engineering, Toyo University

Keywords: 15-acetyldeoxynivalenol, deoxynivalenol, HPLC, LC-MS, multifunctional column

JOURNAL FREE ACCESS

2019 Volume 69 Issue 1 Pages 15-17

DOI https://doi.org/10.2520/myco.69-1-2

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Abstract

A 15-acetyldeoxynivalenol (15-ADON)-chemotype strain of Fusarium graminearum was grown on rice flour solid medium, and 85% acetonitrile extracts of the metabolites were analyzed by HPLC-UV or liquid chromatography-mass spectrometry (LC-MS) with or without pretreatment with a multifunctional clean-up column. Three-way analysis of variance demonstrated no significant differences in the measured levels of trichothecenes irrespective of the analytical instruments used; however, slightly but significantly elevated values were obtained with column pretreatment.

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