JSM Mycotoxins
Online ISSN : 1881-0128
Print ISSN : 0285-1466
ISSN-L : 0285-1466
Letter
Comparison of HPLC-UV and LC-MS methods for evaluating the amount of deoxynivalenol-type trichothecenes in axenic solid culture of Fusarium graminearum
Akira TanakaKoki ShinkaiKazuyuki MaedaYuichi NakajimaShigeru IshiiYasuhiko YoshidaMakoto KimuraNaoko Takahashi-Ando
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2019 Volume 69 Issue 1 Pages 15-17

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Abstract

  A 15-acetyldeoxynivalenol (15-ADON)-chemotype strain of Fusarium graminearum was grown on rice flour solid medium, and 85% acetonitrile extracts of the metabolites were analyzed by HPLC-UV or liquid chromatography-mass spectrometry (LC-MS) with or without pretreatment with a multifunctional clean-up column. Three-way analysis of variance demonstrated no significant differences in the measured levels of trichothecenes irrespective of the analytical instruments used; however, slightly but significantly elevated values were obtained with column pretreatment.

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© 2019 Japanese Society of Mycotoxicology
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