Purity determination of natural toxin standards by NMR spectroscopy

Abstract

  Accurate quantity of the diarrhetic shellfish poisoning Okadaic acid and Ciguatoxins which are the causative substance of ciguatera fish poisoning were determined by qNMR. The signals for use of determining quantity were carefully chosen because both substances are large polyether cyclic compounds. An internal standard material enable to be removable after measurement was used for quantification of the precious marine biotoxins. The uncertainty estimated for the quantitative values of both substances increased with decreasing sample amounts for measurement. Quantification of cyclic polyether compounds having molecular weight around 1000 was achievable for subjects at least 0.01 mg by employing qNMR .