Detection of ochratoxin A and T-2 toxin by one step ELISA

Abstract

A simple and improved enzyme-linked immunosorbent assay, one step ELISA, for quantitation of ochratoxin A and T-2 toxin was developed by the utilization of horseradish peroxidase (HRP) labelled anti-toxin monoclonal antibody on 96 well immunomicroplate. One step ELISA is not only handy method but also considerably improved one with respect to the sensitivity for the toxins compared with indirect (or two step) ELISA. The detection limits for ochratoxin A and T-2 toxin were ca. 50 pg and 5 pg/assay, respectively.