Nano Biomedicine
Online ISSN : 2185-4734
Print ISSN : 1883-5198
ISSN-L : 1883-5198
ORIGINAL ARTICLES
Characterization of the Mechanism by Which Papain Suppresses Tooth Discoloration
Yoshihiro YOSHIKAWAAkari TERAMOTOAyano NISHIDAEma OKAMOTOHiroko KINOSADAWakana SUGIMOTOTakeshi HIROSETsuyoshi SHIMAOKAAiko KAMADAEisuke DOMAEYuya HIRAISusumu TSUDATakashi IKEO
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キーワード: papain, saliva protein, hydroxyapatite
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2017 年 9 巻 2 号 p. 83-88

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Interest in esthetics has increased in recent years, and demand for tooth coloration treatments is increasing. Since proteolytic enzymes, such as papain (papaya proteinase I), have been used in toothpastes to prevent tooth discoloration, this study investigated the mechanism by which papain prevents tooth discoloration. Various concentrations of papain (0.005, 0.05, and 0.5 mg/mL) were added to saliva, salivary proteins were subjected to electrophoresis, and changes in protein properties were examined by Coomassie Brilliant Blue staining. In addition, saliva specimens containing hydroxyapatite (HA) powder were mixed with papain (0.5 mg/mL) to examine changes in the proteins adsorbed onto HA. As a result, papain was found to degrade salivary proteins in a concentration-dependent manner. Papain also degraded salivary proteins that had adsorbed onto HA. Treatment with papain suppressed discoloration by decomposing the pellicles on tooth surfaces.

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© 2017 Nano Biomedical Society
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