2016 Volume 23 Issue 1 Pages 14-20
For neuropathological diagnosis of glial tumors, molecular diagnosis is also important as well as classical histological diagnosis. Key molecules for glial tumors include mutations in isocitrate dehydrogenase (IDH) 1and 2, 1p/19q loss of heterozygocity (LOH), p53 mutation, ATRX mutation, MGMT promoter methylation, Tert promoter methylation as well as Ki67. Immunohistochemistry is simple, robust and universal detection methods for these mokecules compared to genetic analysis. We applied automatedquantitative analysis for detection of Ki67 index, p53 mutation and MGMT promoter methylation using Gunma-LI that were developed for Ki67 detection of brain tumors. Automated count (trial method) and manual count as a golden standard) of 18 samples of glioma sections were compared. We found automated analysis with Gunma-LI is useful for quantitative detection of p53 mutation and MGMT promoter methylation as well as Ki67 index.This method might be applied for individual molecular analysis instead of genetic examination in multicenter clinical trial.
