The effects of long-term low-dose EHDP on experimental osteoporosis induced by ovariectomy and low calcium diet in rats have been investigated. EHDP was given at a dose of 0.5mg/kg daily for six months. Low calcium diet containing 0.1-0.2% Ca, 0.65-0.80% P and 2000 IU vitamin D3/kg was used.
1) Osteoporosis induced by low calcium diet was more marked than that of ovariectomized rats.
2) Both osteoporosis were clearly prevented by EHDP, judging from increased trabecular bone mineral content, cortical thickness, bone ash content and bending strength.
3) These findings suggest that long-term low-dose EHDP can prevent the osteoporosis induced by ovariectomy and low calcium diet as well as the denervation osteoprosis.
