グルクロン酸分離定量法について
1958 年 55 巻 2 号 p. 92-102
詳細
1958 年 55 巻 2 号 p. 92-102
A quantitative method is proposed in order to estimate free glucuronic acid and conjugated glucuronide separately.
The principle of this method isthat free glucuronic acid is reduced to non-chromogenic substance, while conjugated glucuronide remained in tact at appropriate cohdition including the addition of asmall amount of sodium borohydride.
The procedure is as follows: 3mg of sodium borohydride is added and is kept for 15 minutes at 24°C; N/10 NaOH is added to another aliquot and heated for 10 minutes to destroy ester glucuronide;then, colorimetry is carried out by Dische's carbazol method for each specimen.
Four crystalline glucuronide sample were obtained from rabbits' urine: p-dimethylaminobenzoylglucuronide and p-aminobenzoyl glucuronide as ester conjugate, and borneol glucuronide and p-menthan3, 8-diolglucuronide as ether conjugate.
By using these substances, the above proposed method proved to be excellent to differenciate free and each conjugated form of glucuronic acids.
