ヒト生検胃粘膜の組織化学的研究
Phosphorylaseについて
1966 年 63 巻 9 号 p. 975-992
詳細
1966 年 63 巻 9 号 p. 975-992
Activity of polysaccharides synthesis of the stomach mucosa cells in normal stomach andchronic gastritis was studied histochemically by means of phosphorylase-reaction. Phosphoricacid glucose was utilized as the basic substance for the polysaccharides synthesis and phosphorylase-reaction was mainly used by iodine method added with alcohol.
The carbohydrates originally occurring in the stomach mucosa cells was easily differentiatedby the color change. The materials wer human biopsy specimen of the stomach and alsonormal mouse stomach mucosa as the basic experiment.
The results were as follows:
1) In the normal stomach mucosa, an activity of brown purple red reaction thought to bedue to glycogen type was present at the base of the surface epithelium and this proved a presenceof medium degree of activity of the synthesis. In the pathological status of the stomachmucosa the activity fluctuated in the chronic gastritis, slightly increased in the presence ofthe superficial change of the mucosa, decreased in the atrophic change and progressivelydecreased as intestinal metaplasia progressed.
2) A slight activity thought to be amylose type was noted in the smooth muscle fibers ofthe stroma and the blood vessels. There was no appreciable quantitative difference accordingto the areas of the stomach and also degrees or the types of the gastritis.
3) No phosphorylase activity was noted in the gland cells.
4) In the lamina muscularis mucosae, an intensive activity of the amylose type was notedand it was clearly shown that the polysaccharides synthesis was important for the source ofenergy of the muscle contraction in every case of the chronic gastritis.
5) It was understood that metabolic cycle of glucose-6-phosphate was actively present inthe presence of superficial change of the stomach mucosa.
This phenomenon was speculated as to relate the regeneration and proliferation of themucosal epithelium.
This phenomenon was speculated as to relate the regeneration and proliferation of themucosal epithelium.
6) In a necrotic and metaplastic tissue, phosphorylase activity was reduced or disappeared.
7) In the area of intestinal metaplasia, the histochemical changes were similar to that ofintestinal chorioepithelium.
