Normal white male rats, weighing about 200gm., were fed on the diet composed of 0.06% 3' Methyl 4 dimethylaminoazobenzene with basal diet.
Rats were killed at 4, 8, 12 and 16 weeks after beginning of feeding of this diet, and the livers were removed for succinic dehydrogenase (SDH), Diphosphopiridine Nucleotide diaphorase (DPN), Adenosine Triphosphatase (ATP) and Hematoxyline and Eosine stain. The liver obtained from the rat after 4 weeks feeding showed marked proliferation of oval shaped cells at the portal space of the liver and which showed little activities of SDH and DPN, although the parenchymatous cells showed regeneration but normal zonal difference and normal activities of these enzymes.
After 8 or 12 weeks of feeding these oval shaped cells assumed the shape of grandular architecture surrounded by proliferating connective tissue which showed significant ATP activity, and some of the cells which formed the grandular architecture showed irregular in size with significant SDH and DPN activity in the cytoplasma. After 16 weeks of feeding, Those cells which aquired significant SDH and DPN activities became hepatocellular carcinoma and the cells which did not aquire any those activities became cholangiocellular carcinoma.
The SDH and DPN activities of the hepatocellular carcinoma were decreased with the grow, and the activities were then located at the cell memblane of the hepatocellular carcinoma.
