LYMPHOCYTE THAT PRODUCES CHOLESTATIC FACTOR AND MACROPHAGE ACTIVATING FACTOR DIFFERS FROM THE ONE THAT PROLIFERATE IN RESPONSE TO ANTIGEN

Abstract

When the lymphnode cells from the sensitized guinea pigs were stimulated with the specific antigen in vitro, both lymphocyte transformation and production of lymphokines, such as choles-tatic factor and macrophage activating factor were induced. However, lymphocyte transforma-tion was clearly blocked by treatment of cells with bromodeoxyuridine followed by visible light radiation. In contrast, the productions of both cholestatic factor and MAF were not affected but rather enhanced by such bromodeoxyuridine treatment.
These results suggest that lymphocyte that produces cholestatic factor and MAF differs from the cell that proliferate in response to antigen, and indicate that lymphocyte transformation may not be prerequisite to the production of lymphokine.