Eight species were recorded for the first time in the Shimoyama River system. The survey was conducted between September and October 2016 in the lower reaches of the Shimoyama River and in one of its tributaries, the Yokote stream. Five tropical/subtropical species (Lutjanus argentimaculatus, Eleotris melanosoma, E. fusca, Redigobius bikolanus, and Scatophagus argus) were collected in its lower reaches of the Shimoyama River. Rhinogobius mizunoi and E. oxycephala, listed as near threatened (NT) and endangered (EN) species in The Red Data Species in Kanagawa Prefecture 2006, respectively, were also obtained in the Shimoyama River system. In addition, the photographic record of E.acanthopoma from the lower reaches of the Shimoyama River in September 2013 was also described.