New Model of Experimental Hydrocephalus (Part I)

—Its Preparation and Histological Findings—

Abstract

A new model of experimental hydrocephalus was prepared by innoculation of KS-1 cells into the cerebrum of C57 Black mice. KS-1 cells which originated from a human glioblastoma multiforme were first established in 1969 by the authors. The cells have undergone 150 passages.
Doses of 0.03 ml of KS-1 cell solution (10⁶ cells/ml) were innoculated in the right temporal lobes of 63 ten day-old C57 Black mice. Two to four weeks after the innoculation, hydrocephalus developed. Incidence of hydrocephalus was 17 mice or 27%. In histological studies, no obstruction was observed in the CSF system. The lining of the ependymal cells was stretched and interrupted. In the subependymal tissue, spongy formation and gliosis were observed. The subarachnoidal space was widened to some extent and contained some hemorrhaging.