Abstract
The effect of human recombinant interleukin-1β (hrIL-1β) on tumor growth was studied in eight glioma cell lines. hrIL-1β inhibited growth in all cell lines, but to varying extents. Two cell lines were suppressed by 0.5 ng⁄ml hrIL-1β, and three cell lines required 20 ng⁄ml. hrIL-1β also induced morphological changes and increased F-actin contents. hrIL-1β-treated cells demonstrated multipolar shapes and numerous processes with a greater number of cell-cell contacts 24 hours after treatment. Fluorescence microscopy revealed that these processes contained a large amount of polymerized F-actin. These results suggest that hrIL-1β-mediated growth inhibition may be related to the differentiation of glioma cells.
