Abstract
The MICHAELIS constant of catalase-H2O2 system was determined by both methods, MICHAILIS-MENTEN'S and LINEWEA.VER-BURK'S, as 0.15 and 0.35 mole at 0.5°C respectively and the validity of the latter value was postulated. The MICHAELIS constant decreases with ascension of temperature. The heat of dissociation of the intermediate compound was calculated as 3, 100 cal./mole, which was compared with the apparent activation energy, 3, 700 cal./mole between 0.5 and 10.5°C. It was conclusively discussed that the MICHAELIS' theory, in opposition to CHANCE's opinion, was also valid to the catalase-H2O2 system.
