Studies on the Thermophilic Cellulose-Decomposing Bacteria

Part 1. (Supplement) Some Informations about Cultural Environments and Isolation Techniques

Abstract

To get a pure culture of cellulose thermophiles, the agar plating method was examined using Na carboxylmethylcellulose, cellodextrin or cellobiose as the carbon source under a suitable, rH and an anaerobical condition with carbon dioxide or nitrogen gas. By this method nearly pure cultures of cellulose thermophiles were obtained, but these failed to digest cellulose and to recover their activity. The filtration method was also unsuccessful, because their sizes were not remarkably different and a homogeneous culture was not obtained owing to their short reproduction time.