日本農芸化学会誌
Online ISSN : 1883-6844
Print ISSN : 0002-1407
ISSN-L : 0002-1407
脂肪酸の分離と決定
(第16報)高級不飽和脂肪酸のペーパークロトグラフィ
井上 吉之野田 万次郎
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1953 年 27 巻 1 号 p. 50-53

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To provide a simple method for the separation and identification of the component fatty acids of fats, the study of the paper chromatography of unsaturated higher fatty acids was made by using the reversed-phase technique which was developed in the preceding paper [Y. INOUYE and M. NODA: This Journal, 26, 634 (1952).]. The RF values for fourteen unsaturated acids given with the suitable solvent systems, methanol-petroleum hydrocarbon or methanol-acetone-petroleum hydrocarbon, were measured. For detection of fatty acids on the chromatograms, a solution of 0.2% bromocresol green in alcohol was generally used, while either 2% iodine in alcohol or WIJS' iodine monochloride solution was useful to detect only unsaturated fatty acids. Therefore by pairing these spray reagents, it becomes easier to distinguish the spots of unsaturated from that of saturated acids on the paper chromatograms.
On the effects of structure of the fatty acids upon the RF values, the following generali-zations can be made in the descending chromatography with methanol-petroleum hydroca-rbon system. Generally, the longer the aliphatic chain of a fatty acid, the lower the RF value. With acids of equal chain length, increasing the number of double or triple bonds and hydroxyl groups also increases RF value: saturated<monoethenoid<diethenoid<triethenoid (conj.)<monoethinoid<triethenoid <hydroxymonoethenoid acids. Differences in RF values between cis- and trans-isomers are slight in general cases.
The method is suitable for the analysis of the component fatty acids of natural fats.

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