Oxidative Phosphorylation in Higher Plants Homogenate

Abstract

A direct decrease in inorganic phosphate under aerobic conditions was observed in a homogenate system from the higher plant, as it has been found in animal homogenates.
Phaseolus Mungo seedlings were homogenated in a solution which consisted of 0.087M succinate, 0.0013M ATP, 0.013M phosphate, 0, 013M MgSO₄, 1.3% glucose, 1.3mg/cc hexokinase and 0.06M NaF. The homogenate obtained was filtered through gauze and shaken in a Warburg appartus at 37°.
An example of the measurement was illustrated in Table 1. Effects of pH of the medium, concentration of homogenate, constitution of the gas phase and the presence of arsenate were given in Fig. 1, Table 2, Fig. 2 and Fig. 3, respectively. The P/O ratio obtained was 0.33 (Table 3).