All issues
Volume 27, Issue 5
Displaying 1-23 of 23 articles from this issue
- |<
- <
- 1
- >
- >|
-
Buji MARUO, Michio MATSUHASHI1953 Volume 27 Issue 5 Pages 215-218
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA direct decrease in inorganic phosphate under aerobic conditions was observed in a homogenate system from the higher plant, as it has been found in animal homogenates.
Phaseolus Mungo seedlings were homogenated in a solution which consisted of 0.087M succinate, 0.0013M ATP, 0.013M phosphate, 0, 013M MgSO4, 1.3% glucose, 1.3mg/cc hexokinase and 0.06M NaF. The homogenate obtained was filtered through gauze and shaken in a Warburg appartus at 37°.
An example of the measurement was illustrated in Table 1. Effects of pH of the medium, concentration of homogenate, constitution of the gas phase and the presence of arsenate were given in Fig. 1, Table 2, Fig. 2 and Fig. 3, respectively. The P/O ratio obtained was 0.33 (Table 3).View full abstractDownload PDF (398K) -
Kiichi IWAMOTO1953 Volume 27 Issue 5 Pages 218-222
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) Further studies on several kinds of strains in rice-fodder such as Natto bacteria belonging to Bacill. subtilis were carried out, but these microbes differed from Actinomycetes in the vitamin B12-producing activity.
(2) The diseased animals were treated by the intermuscular injection of vitamin B12, and the satisfactory response on the variation of red blood cell count and of the diameter of red corpuscles. Therefore, it was found that “Kuwazu disease” may be a form of macrocytic anemia, namely endemic vitamin B12 deficiency in cattle owing to the absence of vitamin B12-forming microorganisms due to the toxic power of SiF6'' ions.
(3) More recently, the presence of Actinomycetes in the rnmen and feces of cattle suffering from “Kiriyoi disease” in Shimane Prefecture was tested. In this case also, no Actinomycetes was detected. On the contrary, control examinations for healthy animals in the province always indicated posiive results.
(4) The curative experiment of “Kiriyoi disease” with vitamin B12 by injection was also successfully completed.
(5) The vitamin B12-deficiency in cattle and biosynthesis in vivo of this vitamin seem to be considered as in Fig. 3.View full abstractDownload PDF (1153K) -
Part 1. The Analysis of Media and Borth in the PlantK. KOSHINO1953 Volume 27 Issue 5 Pages 222-225
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. The acetone yields and the amounts of iron in the broth of acetone-butanol fermentation plant were analysed.
2. The acetone yields were poor when amounts of iron in broth increased.
3. It was found that iron in the broth were originated from digester by cooking of raw materials.View full abstractDownload PDF (347K) -
Part 2. The Influence for the Butanol Fermentation OrganismsK. HOSHINO1953 Volume 27 Issue 5 Pages 225-228
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. The inhibiting substance which decrease the acetone yield was produced by cooking of molasses with iron and ferrous salts.
2. The cultivation of organisms could be reduced the inhibitance.
3. It was found that organisms selected through colonies from one strain exhibited the difference of resistance against the inhibition.
4. The difference of resistance in one strain to the inhibition were considerably fixed genetically.View full abstractDownload PDF (357K) -
Part 7. Induction of Induced Mutations in Aspergillus sojae by Nirogen Mustard (1)Nobuyoshi IGUCHI1953 Volume 27 Issue 5 Pages 229-233
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) This investigation is concerned with the mutagenic action of the nitrogen mustard, methyl-bis-beta-chloroethylamine on the Aspergillus sojae of soy making. A study was made of morphological mutations.
(2) It was found that nitrogen mustard treated spores germinated slower than the controls, which germinated within 48 hours in the case of untreated 7 days old spore.
(3) It was found that spores of Aspergillus sojae were susceptible to nitrogen mustard, namely almost all the spores were killed at 0.01M concentration in 20 minutes but they were not killed at 0.005M concentration completely in 30 minutes.
(4) The mutation rate increased linearly with nitrogen mustard treatment as in X-ray irradiation and mutation rate of approximately 30% were obtained at 0.01M concentration, but in the case of ultraviolet radiation mutation rate reached a peak and then declined. Nitrogen mustard was recognized as very effective in producing mutations as in the case of ultraviolet.
(5) The author observed in this experiement as follow &: Lots of olive; light, sterile and restricted types were obtained as mutant. In almost cases light type strains were unstable, but olive type strains were rarely unstable. There was no evident connection between morphological mutation ratio and frequencies of mutant types. Frequencies of biochemical mutants were extremely few.View full abstractDownload PDF (474K) -
Part 1. GlucomannanKaneo HAYASHI, Yukio NAGATA, Takashi MIZUNO1953 Volume 27 Issue 5 Pages 234-236
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe mucilage is isolated from cold water extract of the bulb of Lycoris radiata HERBERT by adding the Fehling's solution. And it is confirmed that this mucilage is a glucomannan consisted of one part of glucose and four parts of mannose.View full abstractDownload PDF (277K) -
Part 5. On FerritinMasao KANAMORI, Osamu TANAKA, Jun NAKAGAWA1953 Volume 27 Issue 5 Pages 236-239
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe crystalline ferritin was isolated by means of fractionated precipitation of (NH4)2SO4 after water extracting the horse-spleen-protein with warmed at 50° for 10 mins., and its yield was about 0.15% of fresh matter. The isoelectric point of ferritin was about pH 4.4, and the maximum absorption band of it was at 280 mμ. The content of iron in ferritin was 20.5% per protein, and it was realized that the iron content of it was variable by the state of sample and its treatment, and that the ferritin was homogeneous electrophoretically, but in acid side under the pH 3.6 its descending patterns were heterogeneous. The spreadings which produced in this experiment were also discussed.View full abstractDownload PDF (321K) -
Part 2. Mode of the Action of Irpex-cellulase upon its SubstratesKazutosi NISIZAWA, Toshio KOBAYASHI1953 Volume 27 Issue 5 Pages 239-242
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe investigation in the mode of the action of cellulose-splitting enzymes, by which polysacharides having 1, 4 β-glucosidic linkage are broken down finally to glucose, has been carried out. The results obtained are summarized as follows:
(1) It was confirmed by measuring the reducing at'ility, the rotatory power and the RF value, that the split sugar out of hydrocellulose by a cellulase solution freed from β-gluco-sidase and cellobiase was almost all cellobiose except very small amount of, glucose, and moreover the cellobiose was isolated in crystalline fofm from the sugar solution.
(2) The ratio of the copper numbers of different, , kinds of hydrocelluloses before and after the enzymic attack is almost inversely proportional to the weight ratio of these specimens.
(3) The ratio of the iodine values and the weight of those different kinds of cellodextrins before and after the enzymic breakdown, which should be the degree of polymerization of 8-12, are also inversely proportional to each other.
(4) In view of these results it is infered that cellulase cuts off successively cellobiose from the end portions of the long chain of cellulose.View full abstractDownload PDF (381K) -
Part 3. Specificity Limit of Cellulase to β-Glucosidase in Irpex lacteusKazutosi NISIZAWA, Toshio KOBAYASHI1953 Volume 27 Issue 5 Pages 242-245
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe action of cellulase and β-glucosidase (=cellobiase) in Irpex lacteus upon p-nitrophenol-β-cellobioside was observed, in order to investigate the specificity limit in the splitting of their substrates. It was found by an estimation of the reducing sugar and the separated aglucone in the reaction mixture of each enzyme, that this β-cellobioside was split into glucose and p-nitrophenol by β-glucosidase and, moreover, it was also broken down by cellulase in such manners as glucose+p-nitrophenol-β-glucoside and cellobiose+p-nitrophenol.View full abstractDownload PDF (385K) -
Part 6. Studies on the Measuring of Chelation Intensity of Fusarinic acid and its Analogous Chelation Substances with Cu.. or Fe... by Polarographic MethodKinjiro TAMARI, Jun KAJI1953 Volume 27 Issue 5 Pages 245-249
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) We measured the chelation intensity of fusarinic acid and its analogous chelation substances with Cu.. or Fe... bya polarographic method. The order of the chelation intensity of these substances is as follows; Fusarinic acid>α-picolinic acid 2-methylpyridine-4-carboxy-lic acid>fusarinic acid ethylester>nicotinic acid; and this order is identical with that of the injurious actions of these substances on plant growth.
(2) We measured the diminution of Fe... -ion by a polarographic method in the test with rice seedlings cultured on the cotton wool, which absorbs respectively the solutin of above mentioned substances and found that, the more chelation of substance is strength the more Fe... -ion in rice plant diminute.
This fact shows that the mechanism of fusarinic acid's injurious action on Fe-porphyrin-enzyme, such as cytochrome, catalase, peroxidase, is, ascribable to the chelation of this acid.
(3) We measured the power of chelation power of various heavy metal-ion with fusarinic acid.
The order of the chelation power of heavy metal-ion is as follows; Fe...>Cu..>Cd..>Ni..>Co..
The chelation power and the reduction-potential of heavy metal-ion are inversely proportional.View full abstractDownload PDF (1180K) -
Part 7. Studies on the Elimination of Injurious Action of Fusarinic Acid on Plant-growth by Addition of Heavy Metal-ionKinjiro TAMARI, Jun KAJI1953 Volume 27 Issue 5 Pages 249-252
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSWe studied on the elimination of the injurious action of fusarinic acid on rice seedlings' respiration and the power of catalase and peroxidase. We found the injurious action of this acid is to be eliminated partially by thee addition of equivalent normal of Fe... or Cu.. and then almost all by the addition of Fe... together with a slight amount (6/1000, 000) of Cu.., Mg.. and Mn..View full abstractDownload PDF (864K) -
Isolation of Japanese Soil Streptomycetes and its Bacteria SpectrumKoiti NAKAZAWA, Shigehiro FUJII1953 Volume 27 Issue 5 Pages 253-255
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) 2350 strains of Streptomyces were isolated from soil of many places in Japan.
(2) The casein-agar was found to be the most suitable medium for the selection.
(3) By the Cross Streak Method, 39% of the strains isolated were active against test organisms and 61% were not.View full abstractDownload PDF (256K) -
Part 3. Intermediary Production of 2-Hydroxyphenylacetic Acid on the Phenlacetic Acid Metabolism. (1) Isolation and Identification of 2-Hydroxyphenylacetic AcidMasao ISONO1953 Volume 27 Issue 5 Pages 255-259
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) During the cultivation of Penicillium chrysogenum Q-176 in the synthetic medium containing phenylacetic acid (PAA), the author noticed the presence of a phenolic compound which was positive for FeCl3 reaction or Millon reaction, and of a ketone body which was positive for Rothera's nitroprusside reaction in the culture broth.
(2) The suitable synthetic medium was selected, in which a good deal of the phenolic compound and ketone body were accumulated.
(3) From the result of titration of, benzene and ether soluble organic acids in the culture broth, it was presumed that the phenolic compound was an intermediary metabolite of PAA.
(4) The phenolic compound, which was a metabolite of PAA, was isolated as crystal from the 72 hour culture broth after the addition of PAA. Method of isolation of this compound was summarized in Fig.l. Judging from its properties such as the m. p. (146°), the analysis of elements and the absorption spectra coupling with FeCl3 and indophenol reagent (2, 6-dibromoquinonechloroimide), this compound was identical with 2-hydroxyphenylacetic acid.
(5) The amount of succinic acid isolated from the culture broth containing PAA was greater than that which was isolated from the culture broth without PAA. From this reason, succinic acid was presumed as a metabolite of PAA.View full abstractDownload PDF (431K) -
Part III. Intermediary Production of 2-Hydroxyphenylacetic Acid on The Phenylacetic Acid Metabolism. (2) Oxidative Metabolism of 2-Hydroxyphenylacetic AcidMasao ISONO1953 Volume 27 Issue 5 Pages 260-264
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) A few oxidative natures of 2-hydroxyphenylacetic acid (2-OH-PAA) were examined manometrically. The oxidative activity of 2-OH-PAA was nearly constant between pH 5 and 8; it was not inhibited below the pH (5.6) at which the oxidative activity of phenylacetic acid (PAA) fell off.
(2) For the oxidation of 2-OH-PAA, the optimal concentration of the substrate was above 0.03M; the oxidative activity of 2-OH-PAA was not inhibited above the concentration (0.03M) at which the oxidative activity of PAA was poisoned.
(3) When 1 molecule of 2-OH-PAA was oxidized, 4-4.5 molecules of oxygen were consumed and 3_??_4 molecules of carbon dioxide were excreted. The amount of oxygen consumption per 1 melecule of 2-OH-FAA was less than that of PAA by about half a molecule, and the amount of carbon dioxide output was equal to that of PAA.
(4) By means of Gibbs' indophenol reaction, the change of 2-OH-PAA content was examined in the synthetic medium containing PAA. 2-OH-PAA appeared according to the PAA metabolism, and its maximum content was achieved at about 130 hour culture period, and afterwards, it disappeared gradually with the progress of cultivation. In case of the medium containing 2-OH-PAA instead of PAA, the rate of 2-OH-PAA metabolism was slower than that of PAA metabolism.
(5) The penicillin G formation was examined on the cultures to which had been added PAA and 2-OH-PAA individually as precursor. In case of 2-OH-PAA, the corresponding new biosynthetic penicillin was not found, but it acted as a precursor of penicillin G. From this reason, it was presumed that the enzymatic conversion from PAA to 2-OH-PAA was a reversible reaction.View full abstractDownload PDF (452K) -
Part 2. Effects of Milk Components and Some Emulsifiers on Vitamin A in MargarineKoji NAITO, Takajiro MORI1953 Volume 27 Issue 5 Pages 265-268
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAs previously reported, the skim milk is very effective in preventing the deterioration of vitamin A which is to added to margarine. In this paper, the main components of skim milk were examined on their protecting powers. Proteins, such as casein, albumin and globulin were proved to be very effective, where as lactose and the ash of milk promoted the oxidation of vitamin A conversely.
From the above mentioned results, several water-soluble proteins of another origins, such as casein, albumin, globulin, gelatin and gluten were examined and all of them were confirmed to be effective.
It was supposed that these proteins improved the emulsion of margarine and that the improved state served the protection of vitamin A from the oxidation. However, monoglyceride, a fat-soluble emulsifier, showed little protecting power and when it was used in high concentration, the vitamin A oxidation increased inversely. Therefore, it is not obvious whether the state of emulsion of margarine has anyting to do with the preservation and deterioration of vitamin A or not.
The mechanism of protection of vitamin A by these proteins is now under investigation. Gelatin was more effective than the ordinary emulsifiers, monoglyceride, lecithin and combination of them.View full abstractDownload PDF (359K) -
Part 3. Effects of Watersoluble Colloidal Substances on Vitamin A in MargineKoji NAITO, Takajiro MORI1953 Volume 27 Issue 5 Pages 268-272
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSWater-soluble colloidal substances such as gelatin, casein, sodium alginate, agar-agar, starch, gum arabic and tragadanth were examined on their effects on the preservation of vitamin A which is added to margarine and found to be effective except starch and tragacanth. Generally, the water-soluble proteins were more effective than the colloidal carbohydrates.
Both 0.5 or 0.1%, of sodium caseinate and combination of 0.5% gelatin and 0.01 or 0.005 propyl gallate were more effective than 0.02% propyl gallate in margarine.View full abstractDownload PDF (445K) -
Toshio NAKABASHI1953 Volume 27 Issue 5 Pages 272-274
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSFor the micro-estimation of substances in the spots on paper chromatogram, I have extr acted the solute by means of the improved apparatus (Fig. 1, 2. ).
A cut spot of paper chromatogram (P) is inserted in the glass tube, in which there is a paper bridge (B) in order to regulate the rate of extraction, and is washed with the suitable solvent (S).
I have estimated the flavonoide pigments by this apparatus.View full abstractDownload PDF (256K) -
Toshio NAKABAYASHI1953 Volume 27 Issue 5 Pages 274-276
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe existence of rutin in tea leaves was expected by the method of paper chromatography and crystalline flavonoide pigment wnich wao rutin was obtained from the ethyl acetate extraction of tea leaves (Assam sp.).
By means of aluminium chloride and improved apparatus for the extraction of paper chromatographied substance, the total flavone and rutin in several kind of tea leaves have been estimated (Table 1, 2.).View full abstractDownload PDF (272K) -
Part 9. On High Boiling Point's Fraction (4) Isolation of Phenolic Flavorous SubstancesTamotsu YOKOTSUKA1953 Volume 27 Issue 5 Pages 276-281
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSHydroxy-2-methoxy-4-ethyl-benzene was isolated as phenylurethane (mp. 104°) from the steam distillate of Soy-cake, and has proved to be one important component of Soy-flavors.View full abstractDownload PDF (586K) -
Part 2. The Action of Vitamin C and allied substances on the Papain Activity [1]Y. MATSUYAMA, T. MATSUMOTO, M. SOEJIMA, K. SHIMURA1953 Volume 27 Issue 5 Pages 281-285
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) The effects of asorbic acid and allied substances on papain activity was investigated. Dehydro- and monoacetone derivative of ascorbic acid showed strong inhibitory action, while its osazone had no effect.
(2) Methylene blue showed some inhibitory action for papain activity, but the degree of the action was less than ascorbic acid.
(3) Adrenalin, o-quinone, p-quinone and quinhydrone were the most strong inhibitors, followed by gallic acid, digallic acid, pyrogallol, pyrocatechine, hydroquinone. Phloroglucine, and resorcin inhibited papain activity, to some degree, but dimethylhydroquinone, guajacol, thymol and nitranilic acid had no effect.
(4) The inhibition of papain activity by the above mentioned inhibitors was regenerated by H2S.View full abstractDownload PDF (475K) -
Part 3. The Influence upon the Butanol Fermentation Organisms with BacteriophageK. HOSHINO1953 Volume 27 Issue 5 Pages 285-288
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. The inhibiting substance, produced by cooking molasses with iron lowered the acetone yield with bacteriophage compared with bacteriophage only.
2. The inhibiting substance, produced by cooking molasses with ferrous salt seems to increase the titer of bacteriophage.
3. The high acidity organism in butanol producing strain was not affected by bacteriophage which attacked the high acetone yield organism in the same strain.View full abstractDownload PDF (340K) -
Part 4. Selection of Resistant Organisms against Bacteriophage and Inhibiting Substance by Cooking Molasses with IronK. HOSHINO1953 Volume 27 Issue 5 Pages 289-295
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. The LEGG's method of immunization of butai o fermentation organism against bacteriophage was improved and resistant organism was selected from the strain T-9.
2. The resistance of this organism under the existence of iron as an inhibiting substance was examined.
3. The resisting organism against bacteriophage and the inhibiting substance containing iron was selected from strain 314 by using the media which contained an inhibiting substance and bacteriophage.View full abstractDownload PDF (2148K) -
Hiroshi OKAZAKI1953 Volume 27 Issue 5 Pages 296
Published: 1953
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (51K)
- |<
- <
- 1
- >
- >|