Studies on Form of Iron and Iron-containing Material in Living Tissue

Part 10. On Ferritin [4]

Abstract

(1) The approximate R_F values of the individual DNP-derivatives of 19 amino acids in the following several solvents such as propanol-cyclohexane, propanol-ligroin, ethylacetate, butanol, amyl alcohol and ethanol-benzylalcohol have been determined. Propanol-ligroin=70:30 (v/v) has been the most suitable solvent for paper chromatography of DNP-amino acid.
(2) The seven amino groups of alanine, arginine, glycine, histidine, lysine and methionine have been recognized as the free-amino-groups of horse-splesn ferritin and apoferritin, by the method of DNP-amino acid paper-chromatography.