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Volume 28, Issue 9
Displaying 1-24 of 24 articles from this issue
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Part 13. Syntheses of Some ω, ω'-bis-(2, 3, 4-trihydroxyphenyl) Alkanes and their Antioxidant ActivitySaburo TAMURA, Kazuhiko OKUMA, Hide OKUBO1954 Volume 28 Issue 9 Pages 679-682
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSSucceeding to the fact that some of ω, ω'-bis-(3, 4-dihydroxyphenyl) alkanes showed marked antioxidant activity(2)(3) ω, ω'-bis-(2, 3, 4-trihydroxyphenyl) hexane and decane were prepared and their activity was examined in the present paper.
The syntheses of the alkanes: Pyrogallol trimethylether was condensed with adipyl chloride in the presence of AlCl3 to give 1, 4-bis-(2-hydroxy-3, 4-dimethoxybenzoyl) butane (IV), which. was reduced by WOLFF-KISHNER Method to 1, 6-bis-(2-hydroxy-3, 4-dimethoxyphenyl) hexane (VI). (VI) was then demethylated with HI to yield 1, 6-bis-(2, 3, 4-trihydroxyphenyl) hexane (VIII). 1, 10-Bis-(2, 3, 4-trihydroxyphenyl) decane (IX) was prepared, in the same way using sebacyl chloride and pyrogallol trimethylether as the starting materials.
The antioxidant activity of the alkanes: The activity of the compounds, (VIII) and (XI), was examined by the Active Oxygen Method using lard as the substrate. It was found that their activity was superior to those of NDGA and some ω, ω'-bis-(3, 4-dihydroxyphenyl) alkanes as shown in Table 1.
The authors wish to express their appreciation to Professor Dr. Y. SUMIKI for his guidance and advice.View full abstractDownload PDF (380K) -
Hiroshi SUGISAWA, Kiyoshi ASO1954 Volume 28 Issue 9 Pages 682-684
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(a) By the reaction of furfural and liquid-ammonia at a low temperature (10, 6 Atm. ), hydro-furamide (I) (m.p. 117_??_120°) and furfurin (II) (m.p. 116_??_117°) were obtained.
(b) By the reaction at a high temperature (100°, 60 Atm.), the main portion of the product was furfurin, the other was C11H9O2N (m.p. 167_??_170°). (c) Furfurin was obtained quantitatively by the addition of ammonium chloride to the reaction described above. But pyridine derivatives presumed were not obtained.View full abstractDownload PDF (285K) -
Rinjiro SASAKI, Satoshi ODAGIRI, Jyoji YOSHIDA1954 Volume 28 Issue 9 Pages 684-688
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSEffects of V. B12 and liver extract on the destruction of blood-corpuscle in rat injected with phenylhdrazine are experimented. The content of urobilinogen in the excrements is estimated by colorimetric method and the fluctuation of V. B12 content in rat liver is determined by Euglena method.
Blood-corpuscle of albino rat is destructed by the injection with phenylhydrazine. At that time, the injection of V. B12 or liver extract affects on the delay of the destruction of blood-corpuscle, not decreasing the content of V. B12 in the liver.View full abstractDownload PDF (449K) -
Part 3. Main Amino Acids Distribution of Silk Fibroins Degradated with Dilute Acid and AlkaliShuiku SASAKI, Key KOBAYASI, Katzuya HAYASI1954 Volume 28 Issue 9 Pages 688-692
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDeguminated fibroin fibers were partially degradated and stepwisely fractionated with dilute HCl and NaOH, and the distributions of total nitrogen, glycine, alhnine, tyrosine, serine and threonine in these fractions and untreated fibroin were determined. A little heterogeneity of the distributions of these amino acids was observed. This fact might be attributed to the foreign protein which could not be separated from fibroin molecule by the ordinary procedure, or to the shape or construction factors of fibers.
It seems that the mode of peptide linkages of amino acids, various sizes side-chains in fibroin molecules, which have not only factor of so-called crystalline and amorphous regions defined by the treatments with acid and alkali.
The phenomena of the degradation of fibroin fibers, any way, were seemed to be controlled by complicated factors contrasting with cellulose fibers which have simple glucosidic linkages.View full abstractDownload PDF (512K) -
Part 10. On Ferritin [4]Masao KANAMORI, Ichiro MAEDA1954 Volume 28 Issue 9 Pages 692-695
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) The approximate RF values of the individual DNP-derivatives of 19 amino acids in the following several solvents such as propanol-cyclohexane, propanol-ligroin, ethylacetate, butanol, amyl alcohol and ethanol-benzylalcohol have been determined. Propanol-ligroin=70:30 (v/v) has been the most suitable solvent for paper chromatography of DNP-amino acid.
(2) The seven amino groups of alanine, arginine, glycine, histidine, lysine and methionine have been recognized as the free-amino-groups of horse-splesn ferritin and apoferritin, by the method of DNP-amino acid paper-chromatography.View full abstractDownload PDF (397K) -
Akira KAJI1954 Volume 28 Issue 9 Pages 695-699
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA study has been done on the polygalacturonase action of Clost. acetobutylicum E4, Clost acetobutylicum K17, and Cl. butyricum W5. These organisms were useful for the fermentation retting of barks of plant fibre materials. The crude enzyme was prepared from fermentation media of Cl. acetobutylicum E4. When cations in the media were removed by Amberlite IR-120, protopectinase was also taken out of the media. The breakdown of pectic acid was determined by WILLSTÄTTER-SCHUDEL's hypoiodite method and by OSTWALD viscosimeter. When this enzyme acted on pectic acid, its viscosity decreased slightly and its reducing power increased a little. The optimum pH value for this enzymatic action was determined as 6.0 an observation of viscosity and 6.0 to 6.2 by the determination of reducing value. The enzyme was inactivated at 70° when the solution was kept on the temperature for 20 minutes. It was found that nickel, manganese and zinc ions accelerated the action of depolymeric polygalacturonase, when they were added in the concentration of 0.8 gram ion per litre. After this enzyme acted on 0.30% pectic acid solution for 7 hours to. 16 days, D-galacturonic and digalacturonic acids were detected by paper chromatography. Trigalacturoinic acid was not produced except by the enzyme of Cl. felsineum var. sikokianurm W2.
By the results of experiment in which W2 and the three bacteria were compared each other in activity of polygalacturonase, W2 bacteria produced enormously large amount of depolymeric enzyme, W5 a little, E4 and K17 slightly. But saccharifing polygalacturonase was produced a little by W2 and slightly by the other three organisms.View full abstractDownload PDF (480K) -
Part 6. On the Manganese Content of Feeds, especially of the White CloverMasao KAMETAKA, Kiyoo HATAKEYAMA, Ko'ichi TANII1954 Volume 28 Issue 9 Pages 699-703
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) The presence of manganese and its content in feeds, especially in the wild white clover, is observed
(2) The presence of manganese is found at 2794. 8 Å in the spectrogram of the wild white clover ash taken by the uncontinuous arc method of spectrochemical analysis. Besides this, calcium, iron, silicon, magnesium, aluminium, sodium, potassium and copper are also found in the ash.
The contents of iron, aluminium, magnesium and manganese are estimated semi-quantitative-ly. The result shows that they are ca. 1, 0.5, 3.5 and 10-2 in percentage respectively.
Their contents, except manganese, almost coincides with the result of chemical analysis of the ash, already reported in Part 4 of this study by one of the authors.
(3) The manganese content in the wild white clover hay is determined colorimerically by the GOTO-MUSHA's photoelectric colorimeter and the Duboscq colorimeter.
The permanganate colour appears soon after the addition of (NH4)2S2O8 as the oxidising agent, its appearance being catalysed by the silver ion, and is stabilised by the addition of phos-phoric acid.
The absence of mainganesei slicate in the ash is found colorimetrically. The result shows that the manganese content after the treatment with hydrofluoric acid is the same as after the untreatment.
The wild white clover hay contains from 34 to 43 p.p.m. of manganese, which is also checked from the values determined by the Beckman Spectrophotometer at 526 mμ.
In two figures, it is shown that the manganese is precisely determined to the extent of 20γ per cc of the solution by the Beckman Spectrophotometer, though it is scarcely able to be determined over 1γ per cc by the photoelectric colormeter.
The manganese content in several kinds of feed are shown in Table 4.View full abstractDownload PDF (481K) -
Makoto FURUICHI, Tatsuo OKAMOTO1954 Volume 28 Issue 9 Pages 703-707
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIt may be concluded from the investigation of pectic enzymes about various yeasts as follows.
1. Pectin-methylesterase is not produced by yeasts.
2. Yeast pectin-polygalacturonase activity-is heavily affected by the CH3O content of the substrate (pectinic acid). Therefore, the true substrate of yeast pectin-polygalacturonase is pectic acid and this enzyme does not belong to the so-called polymethylgalacturonase reported by SEEGMILLER et al..
3. Pectin-polygalacturonase is widely distributed in yeasts., Saccharomyces fragilis indicates the maximum activity though others generally slight.View full abstractDownload PDF (468K) -
Part 3. Effect of Amino Acids and Vitamins on the Growth of the MyceliumShoji WAKITA1954 Volume 28 Issue 9 Pages 707-711
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. The present study was carried out on the role of amino acids and vitanins upon the growth and fructification of Collybia velutipes (CURT.) QUEL.
2. Collybia velutipes grew vigorously and well fruited on malt extract medium, but very scarcely grew on the synthetic medium.
3. The culture medium for this experiments was the HENNEEERG's synthetic solution containing separately the amino acids of 25 sorts as the special growth substance rather than as the ordinary nutritive substance.
4. Among the amino acids of 25 sorts, l-alanine, l-leucine, l-lysine, cysteine, dl-norleucine, dl-serine, l-histidine, dl-valine, l-phenylalanine, dl-norvaline, l-cystine, isoleucine, l-glutamic acid, dl-tryptophan, proline and dl-aspartic acid were proved somewhat effective for the fungus growth (Table 2).
5. As far as the results of this experiments are concerned, any amino acid did not so much promoted the mycelial growth separately.
6. As to the vitamin the vitamin B group such as vitamin B1, B2, B6, choline chloride, nicotinic acid and calcium pantothenate were added separately.
7. Among them, vitamin B1 and B2 were very stimulus to the fungus growth. When these vitamins were added in the high concentration, V. B1 13_??_1600γ and V. B2 13_??_104γ per 25cc of the medium, the mycelium grew vigorously and its dry weight increased in spite of less coasimption of glucose in the medium (Table 3). Bit the excess of vitamin B1 as much as 1600γ per 25cc of the medium, seemed to prevent the fungus fructification.View full abstractDownload PDF (514K) -
Kin'ichi MATSUSHIMA1954 Volume 28 Issue 9 Pages 711-715
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSBased on the linear relationship between the fungal enzyme concentration and the square transformation of the titration values obtained by the SÖRENSEN's method, a proposed unit of the proteolytic activity is defined as the activity contained by a quantity of the fungal enzyme materials which gives an increase in amino acids released from gelatin in a 10ml aliquot from the digestate corresponding to the intersection of the straight line with the transformed ordinate representing 0.1ml of N/10 alkali, and the unit is suggested to call ‘Gelatin formol unit’View full abstractDownload PDF (478K) -
Hygroscopin, and Antibiotic Substance active against Fungi and PhytopathogensKôiti NAKAZAWA, Kinzo OKI, Isao TADOKORO, Mikio HONJO, Hiroshi H ...1954 Volume 28 Issue 9 Pages 715-716
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) The activity of hygroscopin against fungi, yeasts, phytopathogens, Mycobacterium tuberculosis and Trichophyton was tested.
(2) Hygroscopin is active against fungi, notably phytopathogens.View full abstractDownload PDF (186K) -
Part 2. A Taxonomic Study on the Four Strains of CryptococcaceaeYuwao OHARA, Hideo NONOMURA1954 Volume 28 Issue 9 Pages 717-721
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSFour authentic strains of rather unusual yeasts occuring on a mash and koji of tamari-soya were identified as follows:
Candida polymorpha nov. sp. strain S-3
Cryptococcus diffuens (ZACH) LODDER var. non-membranaefaciens nov. var. strain K 10
Trichosporon behrendii LODDER et VAN RIJ strain K3-3
Candida rugosa (ANDERSON) DIDDENS et LODDER strain K 7
It has been suggested that Cryptococcus diffuens var, non-meinbranaefaciens nov. var. may hage been derived from Rhodotorula glutinis (FRES.) HARRISON.View full abstractDownload PDF (437K) -
Part 3. Saccharomyces rouxii BOUTROUXYuwao OHARA, Hideo NONOMURA1954 Volume 28 Issue 9 Pages 721-724
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIdentification were made on the four etrains of osmophilic yeasts which were most frequently isolated from Tamari-soya-mash. Two of them were recognized as Saccharomyces rouxii BOUTROUX, and other two strains were considered as identical with it.
It is suggested that a peculiar form of cells (Fig. 2 a-h) may be a certain prototype of the pseudomycelium.View full abstractDownload PDF (385K) -
Part 4. On Restoring the Sporulation Ability of Saké-yeastsToraji TSUKAHARA1954 Volume 28 Issue 9 Pages 724-727
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. In order to restore the degenerated or weakened sporulation ability of saké yeasts, the following procedure was found to be recommended: The transplantations of yeasts are repeated 7_??_8 times with intervals of 2_??_3 days and then more 2_??_3 more times with intervals of 7 days (or longer) in the “koji” extract which has not been sterilized by heat but has been freed from microörganisms by filtering with the Berkfeld's filter.
2. The percentage of staining of yeasts with methylene blue increased up to a maximum value on about the seventh day after inoculation and then decreased gradually.
3. The optimum temperature of the preculture to secure the highest sporulation percentage of saké yeasts lays near the maximum temperature of their multiplication.View full abstractDownload PDF (384K) -
(1) On the Mode of Growth Inhibition of Dehydroacetic Acid and the Influence of Medium pH on its Activity (2) On the Elimination of Growth Inhibitory Action by Addition of Thiol-compound or Metal-ionMasao NOMOTO, Mitsuo NAMIKI, Kazuko NAMIKI1954 Volume 28 Issue 9 Pages 727-732
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSGrowth inhibitory actions of both DHA (Dehydroacetic acid) and DAC (Dehyoroacetocarooxylic acid) against several microörganisms were studied by the method of photoelectro-turbidi-metry. The results were as follows:
(1) Both DHA and DAC inhibited the growth of microörganisms in the logarithmic and stationary-phase of their multiplications.
(2) DHA inhibited the growth of yeast and Acetobacter, but its antimicrobial activities disappeared against Lactobacilli. On the contrary, DAC inhibited the growth of Lactobacilli. but became harmless against yeast and Acetobacter.
(3) The antimicrobial activities of both DHA and DAC were influenced by medium-pH, i.e. they strongly appeared in acidic-side of pH of medium, and became weaker in alkalineside.
The effects of SH-compounds (cysteine and thioglycolic acid) or metal-ions on the growth inhibitory actions of DHA and DAC were also studied. As a result, there was no eliminatng action by addition of SH-compounds or metal-ions.View full abstractDownload PDF (575K) -
Part 2. Effects of Dehydroacetic Acid on the Respiration and Fermentation of MicroörganismsMasao NOMOTO, Mitsuo NAMIKI, Kazuko NAMIKI1954 Volume 28 Issue 9 Pages 732-736
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSIn studying the mechanism of antimicrobial actions of DHA (dehydroacetic acid) and DAC (dehydroacetocarboxylic acid), as the first step, we investigated the inhibitory action on the process of provision of energy (respiration and fermentation). Experiments were performed by Warburg's manometric method, using intact cells of several microörganisms. The results were as follows:
(1) In aerobic condition, DHA inhibited the oxidation of glucose, ethanol, pyruvate, acetate by yeast at the concentration of 6.6×10-4M, but didn't injure the anaerobic glycolysis of glucose by yeast even at 3.3×10-3M concentration.
Accordingly, the mechanism of antimicrobial action of DHA against growth of yeast will appear by the inhibition on Cyclophorase system in aerobic condition, and by the inhibition on an unknown stage of metabolism other than glycolysis in anaerobic condition.
(2) As the lactic acid-fermentation of Lactobacilli, DAG was harmless at 3.3×10-3M concentration, so that, the growth inhibitory action of DAC against Lactobacilli would depend on the injurious effect on an unknown stage of metabolism other than anaerobic glycolysis.
(3) The oxidation of glucose by Acetobater was not injured by DHA, but the oxidation of, ethanol was apparently inhibited by DHA at 3.3×10-3M concentration. Since the metabolism of energy-provision of Acetobacter, however, still belongs to the unknown field of our knowledge even today, we could not discuss the cause of the growth inhibitory action of DHA at this step of our investigation.View full abstractDownload PDF (431K) -
Part 3. Effect of DHA on P-turnover during Anaerobic Glycolysis by YeastMasao NOMOTO, Mitsuo NAMIKI, Kazuko NAMIKI1954 Volume 28 Issue 9 Pages 737-740
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe effect of DHA (dehydroacetic acid) on P-turnover during anaerobic glycolysis by yeast (Sac. fteischmann) was investigated using P32 as the tracer. The results were as follows:
(1) DHA (10-3M at pH 5.6) does not interfere the alcoholic-fermentation by yeast's intact cell, but it reducer remarkably the exchange of P with medium as detd. with P32. In other words, it is understood that, DHA releases the conjugation of P-esterifying mechanism from anaerobic glycolysis by yeast as so-called “Uncoupler”
(2) Accordingly, the growth inhibitory action of DHA against yeast is understood to depend on the inhibition on cyclophorase system in aerobic condition, and the injurious effect on the P-esterifying mechanism conjugated with glycolysis in anaerobic environment.
(3) Even though DHA can inhibit the multiplication of yeast but does not interfere with CQ2-production by glycolitic enzyme system. This information is noteworthy in the case of application of DHA to beverage ete.View full abstractDownload PDF (379K) -
M. SATO, T. OUCHI, T. AKATSUKA, A. HIRAMATSU1954 Volume 28 Issue 9 Pages 740-744
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS(1) One of the authors, M. SATO, had once carried out an investigation(1)(2)(3)(4) on the peptidases of green malt, using green malt of Danish barley from the Danish Factories in Salgelse as well as Danish brewery malt from the Carlsberg Beer Breweries and synthesizing, as substrates, LG, LGG, LGGG, AG, and AGG, etc. In the present investigation, the authors carried out experiments on the peptidases, using green malt prepared by the Nippon Beer Bre-weries at Meguro, Tokyo and synthesizing, as substrates, increased number of dipeptides such. as GG, G-l-L, GA, AG, A-l-L, LA, LG, α-amino B. G., and G. α-amino butyric acid.
(2) G-l-L-splitting activity of malt extract (5% glycerin), was gradually decreased during: dialysis at 1°C against 5% glycerin under reduced pressure 80_??_100mm Hg, but was able to, be stabilized for about ore week, by the addition of conc. glycerin, making its final conc. 44%, and by being kept standing at 1°C (cf. Tables Ia and Ib).
(3) Cleavages of various peptides by dialysed malt extract thus stabilized were measured under definite conditions and calculated as percentages to the total splitting value. Remarkable differences were observed among the cleavages of these peptides. (cf. Table 2 and 3).
(4) pH-activity-curves were obtained for XG-l-L and Xα-amino B-G. From these curves, it was shown that the optimal pH is 8.0 for XG-l-L and 8.5 for Xα-amino B-G. (cf. Fig. 1). (cf. author's former findings, opt. pH 8.6 for XLG and 7.8 for XAG).
(5) Activations or inhibitions of some metal ions upon the splitting of various dipeptides were tested.
(a) XG-l-L was remarkably activated by Co++ and Mn++ but inhibited by Zn++, while Mg++ had no influence, in 10-3 M each metal ions. As sulphate; Xα-aminoB-G was activated by neither of these metal ions. (cf. Table 4).
(b) XLG was activated by Mn++, XAG by Mg++ and XGG by Co++, while XGA was not activated by Zn++, in 10-3 M of each metal ions. (cf. Table 5)View full abstractDownload PDF (501K) -
Tetsuro KONO, Keiko SATO, Mizue SUZUKI, Ikuko ISOBE1954 Volume 28 Issue 9 Pages 744-748
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe use of a new automatic apparatus for the micro determination of nitrogen was proposed. The motion of the moving furnace was not started until it was heated, and was stopped when the sample begun to boil or decompose. Two capillary tubes, m1 and m2, (6_??_7cc/min. and 5_??_6cc/min. resp. air passed when 10cm water pressure was applied) and a manometer containing CaC12 soln. was used to detect the change of the pressure in the combustion tube (Fig. 1 and 2). Reduced Cu was placed in a small quartz tube which was separated from the combustion tube by a three way cock. Thus the oxidation of Cu during, the gas exchange was prevented, while the CuO was reoxidized in the process, and the sample was never exposed to the hot gas as it was in Gysel's system (Fig. 2 and 3). The rate of the combustion of the sample at 1030° in CO2 was too slow (Table 1a), insertion of CuO behind the sample gave better results (Table 1b and 2b), but the best results were obtained when the sample in a boat was covered with CuO and burnt at 900° (Table 2c).View full abstractDownload PDF (498K) -
Part 7. Paoduction of Ca-5-Keto-gluconate by Shaking CultureMasataka YAMAZAKI1954 Volume 28 Issue 9 Pages 748-751
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe conditions affecting the production of Ca-5-keto-gluconate from glucose by shaking culture of Acetobacter suboxydans, strain 621, were studied. The relation curve between pH value and the activity of the oxydation of Ca-gluconate to Ca-5-keto-gluconate in cell suspension showed an optimum at pH 4.0. Since the optimal pH for the accumlation of Ca-5-keto-gluconate was very strict, it was suggested that the amount of CaCO3 added should be controlled in order to affect the optimal conditions.
It was shown that suitable amount of fish extract and CaCO3 were 0.5% and 2.45% respectively per 10% glucose (purity 98.5%) media. Yields of 90.2% of Ca-5-keto-gluconate to glucose have been obtained.View full abstractDownload PDF (244K) -
Part 5. Effect of Temperature and pH and a Comparison of Inhibition by Mercaptide-forming Heavy MetalsMasaharu ITO, Michio ABE1954 Volume 28 Issue 9 Pages 751-756
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1) Effect of temperature and pH on the inhibition of sweet potato β-amylase by -ascorbic acid plus Cu++ has been studied. Moreover, these properties of this inhibition have been compared with those of inhibition caused by other mercaptide-forming heavy metals viz. Ag+. HgCl2 and PCMB.
2) As it was reported previously, this inhibition contains two reactions in series, inhibition by cuprous mercaptide formation (I) and subsequent oxidation of mercaptide by atmospheric O2 (II). From the data presented in Fig. 1, it is presumed that reaction (I) occurs instantaneously, and has no temperature coefficient, while reaction (II) is a slow process and requires activation energy.
3) As pH becomes lower, the extent of reaction (I) decreases presumably as the result of lower affinity of Cu+ to -SH groups of enzyme in such a condition. Consequently reaction (II) proceeds at a slower rate. (Fig. 2_??_Fig. 5)
4) Above-mentioned features of this inhibition including reactivation by cysteine, which was reported previously, are quite the same as those in the case of inhibition by Ag+ and differ in all respects from those by HgCl2 and PCMB. These results are summarised in Table 1.View full abstractDownload PDF (568K) -
Isao ONISHI, Masatosoi NAGASAWA, Kyoko YAMAMOTO1954 Volume 28 Issue 9 Pages 756-758
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSThe authors made a experiment on the oxidation of lignin contained in tobacco stalk and stem (N. T. var. Virginica f. Daruma) in an alkali medium with nitrobenzene. Results obtained were as follows;
(1) The results of the determination of lignin were shown in Table 1. The yield of aldehyde from 100.0g of the stalks was 4.411g. and 0.452g from 100.0g of the stems when 10% sodium hydroxide and 60cc of nitrobenzene were used at 160° for 3 hrs.
(2) Vanillin was obtained from the stalks in 0.4% yield.
(3) The oxidation of tobacco stalk and stem lignin gave a mixture of vanillin, syringaldehyde and p-hydroxybenzaldehyde. Adsorption spectra method using Beckmann spectrophotometer was used for the determination. The ratio of p-hydroxybenzaldehyde, syringaldehyde and vanillin obtained from the stalks was 1:4:4, whereas the ratio obtained from stems was 1:1:4.View full abstractDownload PDF (270K) -
Part 1. A Method of Viable Counts of Bacteria from the Culture mixed with Molds and YeastsKenji SAKAGUCHI1954 Volume 28 Issue 9 Pages 758-764
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESS1. To isolate and count the bacteria in the fermenting Soya-Sauce mash (NaCl 18% nitrogen content 1.4%. The spore of Aspergillus sojae is inoculated to the mixture of cooked soy-bean and wheat. After the materials are covered with the mycelia they are soaked in the brine. Natural fermentation goes on for one year with the aid of contaminated yeasts and bacteria) the antifungal antibioticg added media are very useful.
2. Eurocidin 30_??_100 γ/ml, W-2 substance 25 γ/ml, trychomycin 500 γ/ml, added glucose-broth agar can effectively inhibit many molds and yeasts such as Aspergillus, Mucor, Rhizopus, Peni-cillium, Saccharomyces, Zygosaccharomyces, Torulgpsis, and Candida, and no inhibitance is observed in the plate counting of 38 species of bacteria.
3. The Eurocidin media heated at 100°, 15min. and kept for 8-days show no decrease of activity.
4. The activity of Eurocidin is not so affected by pH.
5. The activity of Eurocidin is affected by media, and that is more effective in the glu-cose-broth agar than in Czapek's agar tested with Asp. sojae.
6. Eurocidin 1mg/cc media shows no inhibitance to Bacillus butyricus.View full abstractDownload PDF (551K) -
1954 Volume 28 Issue 9 Pages e2
Published: 1954
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (35K)
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