Biochemical Studies on the Velvet-Mushroom, Collybia velutipes

Part 3. Effect of Amino Acids and Vitamins on the Growth of the Mycelium

Abstract

1. The present study was carried out on the role of amino acids and vitanins upon the growth and fructification of Collybia velutipes (C_URT.) Q_UEL.
2. Collybia velutipes grew vigorously and well fruited on malt extract medium, but very scarcely grew on the synthetic medium.
3. The culture medium for this experiments was the H_ENNEEERG's synthetic solution containing separately the amino acids of 25 sorts as the special growth substance rather than as the ordinary nutritive substance.
4. Among the amino acids of 25 sorts, l-alanine, l-leucine, l-lysine, cysteine, dl-norleucine, dl-serine, l-histidine, dl-valine, l-phenylalanine, dl-norvaline, l-cystine, isoleucine, l-glutamic acid, dl-tryptophan, proline and dl-aspartic acid were proved somewhat effective for the fungus growth (Table 2).
5. As far as the results of this experiments are concerned, any amino acid did not so much promoted the mycelial growth separately.
6. As to the vitamin the vitamin B group such as vitamin B₁, B₂, B₆, choline chloride, nicotinic acid and calcium pantothenate were added separately.
7. Among them, vitamin B₁ and B₂ were very stimulus to the fungus growth. When these vitamins were added in the high concentration, V. B₁ 13_??_1600γ and V. B₂ 13_??_104γ per 25cc of the medium, the mycelium grew vigorously and its dry weight increased in spite of less coasimption of glucose in the medium (Table 3). Bit the excess of vitamin B₁ as much as 1600γ per 25cc of the medium, seemed to prevent the fungus fructification.