On the Sugar Composition of Hydrol from the Enzymatic Hydrolyzate of Starch Part II
Reversion Product from D-Glucose by Crude Glucoamylase
1966 Volume 40 Issue 11 Pages 401-405
Details
1966 Volume 40 Issue 11 Pages 401-405
Product of reverse action of glucoamylase was prepared by incubating 40% D-glucose solution with 0. 5% of crude glucoamylase at 55°C for 72_??_96 hrs, followed by inactivating the enzyme at 75°C for 10 min. By paper chromatography and paper ionophoresis, nigerose, maltose, isomaltose, laminaribiose, gentiobiose, panose, isopanose and isomaltotriose were detected in the reaction product. Reversion product from 650g of D-glucose was fractionated by a Carbon-Celite column using water and 25_??_15% ethanol as successive elution solvents. Further separation of sugar was carried out by thick paper chromatography and also by Magnesol-Celite column chromatography of the acetylated sugars. In this way, isomaltose, nigerose, maltose, gentiobiose and laminaribiose were identified as their crystalline octaacetates. Panose was identified as crystalline panitol dodecaacetate.
These results suggest that the glucobioses and glucotrioses in the hydrol were produced from D-glucose by the reverse action of crude enzyme. The enzyme used in this experiment showed no transglucosylase activity.
