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Volume 40, Issue 11
Displaying 1-9 of 9 articles from this issue
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Hiroyuki HORITSU, Mikio TOMOEDA1966 Volume 40 Issue 11 Pages 387-393
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSA study was made of the action of taka-proteinase on crystalline taka-amylase A. The proteinase was purified by Amberlite IRC-50 from “Taka-diastase”. Taka-amylase A was found to be degraded about 50 per cent by taka-proteinase in Mcllvaine's buffer solution at pH 7.0, 37°C for 24 hours, without any denaturation. The modified takaamylase A had a higher specific activity than the original taka-amylase A, and was homogeneous in electrophoretic and sedimentation analyses. Several physicochemical constants of it were less than those of the original taka-amylase A. By the action of the proteinase, various amino acids and peptides were found to be released from taka-amylase A.View full abstractDownload PDF (1692K) -
Gas Chromatographic Determination of Organophosphorus PesticidesHiroshi TAKEHARA, Takuya TAKESHITA1966 Volume 40 Issue 11 Pages 394-400
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSSuitable conditions on the gas chromatographic determination of 12 organophosphorus pesticides were studied. Shimadzu Model GC-1B gas chromatograph with a hydrogen flame ionization detector was used. Columns used were packed with: 1) 1.5% high vacuum silicone grease (HVSG), 2) 1.5% SE-30, 3) 1.5% SE-52, 4) 1% QF-1, 5) 1.5% neopentylglycol succinate (NGS), 6) 1.5% diethyleneglycol succinate (DEGS). DEGS was the most suitable for separation of the candidate pesticides. Dicyclohexyl phthalate and diphenyl phthalate were used as the internal standard for the quantitative analysis of technical grade of dimethoate and EPN, respectively. The results of the gas chromatographic analyses of dimethoate and EPN were coincided with those of TLC and colorimetric methods. Gas chromatographic analyses are useful for the determination of dimethoate and EPN in the technical products, and desirable conditions are presented.View full abstractDownload PDF (456K) -
Reversion Product from D-Glucose by Crude GlucoamylaseToshiyuki WATANABE, Sugio KAWAMURA, Keiko SUGAWARA, Kazuo MATSUDA1966 Volume 40 Issue 11 Pages 401-405
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSProduct of reverse action of glucoamylase was prepared by incubating 40% D-glucose solution with 0. 5% of crude glucoamylase at 55°C for 72_??_96 hrs, followed by inactivating the enzyme at 75°C for 10 min. By paper chromatography and paper ionophoresis, nigerose, maltose, isomaltose, laminaribiose, gentiobiose, panose, isopanose and isomaltotriose were detected in the reaction product. Reversion product from 650g of D-glucose was fractionated by a Carbon-Celite column using water and 25_??_15% ethanol as successive elution solvents. Further separation of sugar was carried out by thick paper chromatography and also by Magnesol-Celite column chromatography of the acetylated sugars. In this way, isomaltose, nigerose, maltose, gentiobiose and laminaribiose were identified as their crystalline octaacetates. Panose was identified as crystalline panitol dodecaacetate.
These results suggest that the glucobioses and glucotrioses in the hydrol were produced from D-glucose by the reverse action of crude enzyme. The enzyme used in this experiment showed no transglucosylase activity.View full abstractDownload PDF (358K) -
Shiro SUGAWARA, Toshiko DOIHARA, Urako KOBASHI, Yoichi KABURAKI1966 Volume 40 Issue 11 Pages 406-409
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAny method reported hitherto for the quantitative determination of propylene glycol in the shred of finished cigarette was not necessarily applicable. For instance, in case of gas chromatographic method, the blank value as propylene glycol in tobacco shred was neglected, and, in case of column chromatographic method, the data obtained did not give correct value when the tobacco shred contained 5 to 6 times of glycerine than propylene glycol. A new analytical method which gave more accurate value was established. This paper is describing the method of azeotropic distillation of propylene glycol with toluene followed by the periodic acid. This method was acceptably applied to the analysis of the various shreds of finished cigarettes.View full abstractDownload PDF (287K) -
Estimation of Stability of Organophosphorus Pesticide Dust Formulation by Thermally Accelerated Degradation TestHiroshi TAKEHARA, Masato KOTAKEMORI, Takumi KAJIMURA1966 Volume 40 Issue 11 Pages 410-413
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSSome investigations on the accelerated degradation test of the organophosphorus pesticide dust formulations with elevated temperature were carried out. The methyl parathion and the malathion dust, which were formulated with Zeeklite (Commercial name of clay mineral), were stored for 24_??_116 days at 30°_??_70°C and room temperature. After accelerated test, the residual active ingredients were analyzed and the degradation rate was calculated. The degradation rates of the dusts at various temperatures were fitted to Jander's rate equation, when the degradation rate did not yet proceed to 80%.
Jander's rate equation: {1-(1-α)1/3}2=kt
ex. α: reaction rate, k: degradation rate constant, t: reaction time.
Arrhenius' relationships were established between the degradation rate constants and the temperatures, and that the degradation rates of both dusts at the room temperature, which were estimated by Arrhenius' equation, were in good agreement with the experimental results.View full abstractDownload PDF (301K) -
Isao NISHIKAWA, Nobuaki ABE, Kensuke SAITO1966 Volume 40 Issue 11 Pages 414-420
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSEffect of heat upon bovine skimmilk protein, whole casein and whey protein was studied by means of DEAE-cellulose column chromatography. Casein and whey protein solution, dialyzed against pH 7.0, 0.02M phosphate buffer, and skimmilk were heated for 20 minutes at 70, 80, 90, 100 and 120°C.
Heating casein solution at 120°C for 20 minutes caused some changes in the chromatogram compared with that of unheated casein. But no change was observed between heated and unheated when casein solution was heated below 100°C. Whey protein, on the other hand, showed changes even at 70°C heating. Characteristic changes in the chromatogram of heated whey protein compared with unheated were a long tailed peak between as- and β-casein position, and marked increase of alkali-eluted fraction. Heated skimmilk protein also showed the same tendency. Since heating below .100°C did not affect on casein, the changes must depend on denatured whey protein. The increase of adsorbability of heated whey protein to DEAF-cellulose is supposedly due to the change in net charge and size of protein molecules.View full abstractDownload PDF (427K) -
Action of Carrot Exo-polygalacturonase on the Pectic Acids Prepared by Saponification of Pectin with Alkali and with Pectin EsteraseChitoshi HATANAKA, Junjiro OZAWA1966 Volume 40 Issue 11 Pages 421-428
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSGlycosidic bonds of pectin are cleaved in alkaline solutions by way of a transelimination. The rate of the reaction increases with temperature. In the cold, however, the reaction proceeds slowly and the de-esterification of pectin predominates over the transelimination. At 0°C, the latter can hardly occur, while the former proceeds to completion. In this case an unaccountable drop in viscosity is observed. The pectic acid having an unsaturated galacturonic acid unit at the non-reducing end of the molecule, which is a product of pectin transelimination, was resistant to CPG (carrot exo-polygalacturonase) action.
The pectic acid prepared by the saponification with PE (pectin esterase) contained no unsaturated galacturonic acid unit. This was susceptible to CPG action but the degradation was far from complete. From these results it is considered that the incompleteness of hydrolysis of pectic acid molecules by CPG is caused by the presence of this terminal unsaturated galacturonic acid unit together with neutral sugar units. In contrast to the reported partial de-esterification of pectin achieved by PE, almost complete de-esterification was obtained with PE prepared from peels of Citrus Unshiu.View full abstractDownload PDF (514K) -
Eiji NIWA, Hiroo AOKI, Hiroshi TANAKA, Teiichi TAMURA1966 Volume 40 Issue 11 Pages 429-430
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSAllylbenzene polymerized in the presence of aluminum chloride in nitrobenzene at 0°C From reaction mixture two dimers_??_(I), b. p. 112_??_3°C/0.01mmHg, λCHCl3max 260, 270 mμ (log ε 2.9, 2.8); (II), b. p. 148°C/0.01mmHg, λCHCl3max 256, 270 mμ (log ε 3.2, 2.9)_??_ and a trimer _??_(III), b. p. 182°C/0.1mmHg, λCHCl3max 258 mμ (log ε 3.8)_??_ were isolated by fractional distillation. Zinc dust distillation of (I) and (II) gave 1-phenylnaphthalene and 2-phenylnaphthalene, respectively. By this fact along with NMR-_??_(I), τ=9.1 (3H, triplet, J=6.8 c. p. s.), τ=6.0 (1H, triplet, J=6.7 c. p. s.); (II), τ=9.0 (3H, triplet, J=6.8 c. p. s.)_??_ and mass-_??_(I), m/e 28, 29, 207, 208, 236; (II), m/e 29, 207, 236)_??_ evidences, (I) was assumed to be 1-ethyl-4-phenyl-1, 2, 3, 4-tetrahydronaphthalene and (II) to be 1-ethyl-2-phenyl-1, 2, 3, 4-tetrahydronaphthalene.View full abstractDownload PDF (152K) -
Hiroshi FUKAMI1966 Volume 40 Issue 11 Pages R59-R65
Published: 1966
Released on J-STAGE: November 21, 2008
JOURNAL FREE ACCESSDownload PDF (508K)
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