Abstract
The substances which give yellow color in alkaline paste of wheat flour, were able to be extracted only with alkaline methanol. One of the most effective substances was isolated by column chromatography on silica gel and then was purified by thin-layer chromatography. This substance was identified as methyl ferulate, which was supposed to be produced by ester interchange.
Alkaline dough of wheat starch, which contains synthesized ferulic acid ester of soluble starch, showed the same yellowness as that of wheat flour.
From these facts, it was considered that the yellowness of wheat flour dough in alkaline side was due to ferulic acid which would be esterified.