Abstract
Non protein nitrogenous substances in the aqueous extract of KUKO leaves were fractionated by means of column chromatography on ion exchange resin, zone electro-phoresis and cellulose column chromatography. The presence of active principles responsi-ble for acid formation of lactic acid bacteria under a certain condition was examined in each fraction.
Twenty two amino acids including a-aminoadipic acid, α-aminopimelic acid, ethanol-amine, and β-alanine were detected. A mixture of these amino acids showed marked influence on acid formation of lactic acid bacteria, but any single amino acid was less effective in the stimulation of growth as compared with the mixture of amino acids. In a homologous series of monoamino dicarboxylic acids the acid formation was stimulated with the increase in the length of carbon chain.
Adenine, guanine, xanthine, adenosine and an unidentified nucleotide were detected and these nucleic acid derivatives stimulated the acid formation by lactic acid bacteria.
