Abstract
Mutants resistant to 2-fluoroadenine (2 FA) were derived from Corynebacterium murisepticum, Corynebacterium glutamicum, Brevibacterium flavum, Brevibacterium ammoniagenes or Microbacterium sp. by mutational treatment with N-methyl-N'-nitro-N-nitrosoguanidine. Many of the 2 FA-resistant mutants were found to produce adenine and/or hypoxanthine in a culture medium. Among these 2 FA-resistant mutants, C. glutamicum KY-10478 was used to investigate the cultural conditions for adenine production. The strain accumulated adenine at 2.0mg/ml with a medium containing 10% molasses (as glucose).
