Comparative Study of the Chemical Composition of the Cell Walls of Sake Yeasts Aggregating and Non-aggregating with Cellulose

Abstract

Preparations of cell walls were obtained from Sake yeasts aggregating and nonaggregating with cellulose. Comparative studies of components in the walls of both types of strains showed the following results.
For the walls obtained from young yeasts (cultured for 4 days), no significant difference was observed in the contents of glucose, mannose, protein, phosphorus and glucosamine between the strains. In the walls obtained from aged yeasts (cultured for 10 days), the contents of protein and phosphorus were larger and the content of mannose was smaller in aggregating strains. It was found from analysis of fraction A, which was fractionated from the walls with ethylendiamine, that the differences of contents of these components resulted from the outer layer in the walls. There were no striking differences on the amino acid composition between the walls of young cells of any strains, but in the walls of aged cells the proportions of lysine, histidine, arginine and amino acids which liberated ammonia by hydration were larger and those of threonine and serine were smaller in aggregating strains than in non-aggregating strains.