Abstract
The aldehydes are generally used for tanning agents. A modified method for assessing bound form aldehyde in tanned collagen by means of gas liquid chromatography (GLC) was described. The method consists of hydrolysis of collagen tanned with form aldehyde. conversion of the aldehyde into 2, 4-dinitrophenyl hydrazone (DNPH) and direct determination of DNPH by GLC. By this method, about 98% of formaldehyde bound to collagen can be determined.
