The specific activity of myo-inositol 1-phosphate synthase in the milky stage of rice seed reached a maximum on 10 th to 14 th days after flowering. Myo-inositol 1-phosphate synthase was purified from milky stage rice seeds by ammonium sulfate fractionation, DEAE-cellulose column chromatography, gel filtration on Sephadex G-200 and electrophoresis. The specific activity at the final stage became 168 fold.
The optimum pH and temperature for the activity were 8.0 and 50°C, respectively. The activity remained 90 to 100% in a pH range between 7.5 to 9.0 after storage at 4°C for 24 hr and 30% of enzyme activity remained after the incubation at 70°C for 10min. The enzyme was stable for 6 weeks in a frozen state at -20°C.
The partially purified enzyme required NAD⁺ for its activity and NH₄⁺ enhanced the activity twice. The apparent Km value for glucose 6-phosphate was 1.44mm.

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The authors have studied to elucidate the composition of the essential oil of the fruits from Perilla frutescens L. Brit. var. acuta Thunb. Kudo and Perilla frutescens L. Brit. var. acuta Thunb. Kudo f. discolor Makino.
The essential oil was obtained by steam distillation of the fruits respectively. The essential oil was treated with 5% sodium bicarbonate solution, then with 5% sodium hydroxide solution, and was separated into the three fractions: neutral, phenol, and carboxylic acid fractions.
The resulting each fraction was investigated by column chromatography, gas chromatography, infrared spectrum, mass spectrum, nuclear magnetic resonance spectrum and chemical method.
As a result, twenty compounds were detected as the constituents of Penile frutescens L. Brit. var. acuta Thunb. Kudo: α-pinene, camphene, β-pinene, myrcene, limonene, 3-octanol, 1-octen-3-ol, trans-linalool oxide, cis-linalool oxide, linalool, benzaldehyde, 10-pinanal, β-caryophylene, α-humulene, α-terpineol, carvone, perilla aldehyde, 10-pinanol, β-phenyl ethyl alcohol, perilla alcohol, n-C₄-C₈ aliphatic acids; and sixteen compounds were detected as the constituents of Perilla frutescens L. Brit. var. acuta Thunb. Kudo f. discolor Makino: α-pinene, camphene, β-pinene, myrcene, limonene, linalool, β-caryophylene, α-terpineol, carvone, perilla aldehyde, geraniol, benzyl alcohol, β-phenyl ethyl alcohol, 8-methyl-8-nonen-1-ol, perilla alcohol, elemicin and n-C₆-C₁₈ aliphatic acids.

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Surface wax of barley leaves was extracted by immersing them in chloroform or ethyl ether for 10 sec. The solvents were evapolated in vacuo. Yield of the wax was 0.78_??_0.83mg with chloroform and 0.46_??_0.74mg with ethyl ether per gram fresh weight of the leaves.
The wax was fractionated by TLC on silica gel (Wakogel B-5) wedg plates using benzene: n-hexane (1:1) as solvent system. Four prominent bands, located with iodine vapor were scraped from the plates and eluted with chloroform. Each band was analyzed by TLC and IR-spectroscopy.
The wax was shown to contain alkane (4_??_6%), alkyl ester (9_??_11%), alkyl ketone (2_??_5%) and alcohol (60_??_68%). Alcohol, the main component (50_??_55%) of the wax, was identified as 1-hexacosanol by GLC and GC-MS. Acid portion of alkyl ester was composed of the series of even-numbered and unbranched carbon-atom chains from 16 to 26 and alcohol portion consisted of that from n-C₂₀ to C₂₆. Ketone was principally n-C₂₈ homologue. Alkane contained predominantly the series of odd-numbered carbon-atom chains from 25 to 33.

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By using cut potato tuber treated with alkyl halides, experiments were carried out to investigate influences of halogens on rishitin induction and browning in the potato tissues. The results indicated that rishitin was produced higher in tuber tissues treated with alkyl bromides as ethylene dibromide and dibromopropane than these treated with alkyl chloride as ethylene dichloride and dichloropropane. And then, rishitin content was very higher in tuber tissues treated with alkyl halides of having dihalogens than those of having monohalogen. On the contrary, no rishitin was detected in tuber tissues treated with allyl amine and allyl alcohol. On the other hand, there was no relation between the amount of rishitin accumulation and browning intensity in tuber tissues by used alkyl halides.
Tuber tissues treated with alkyl halides, reached maximal rishitin content about 4 days after treatment when treated with EDB and DBCP at concentration of 0.04M, but rishitin induction was strongly inhibited when treated with those at concentration of 0.25M.

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An auto-analyzer of ω-amino acids has been set up by incorporating a reaction system of the phenol-sodium hypochlorite color reaction specific for ω-amino acids into a commercial, multi-line amino-acid analyzer. Conditions of the color reaction has been modified from those of ordinary test-tube reaction so as to obtain reproducible and highly sensitive analyses of ω-amino acids in biological materials by using this apparatus. As applications, free and bound-form ω-amino acids in some wild mushrooms have been analyzed and β-alanine formed by the action of aspartate α-decarboxylase on aspartic acid has been determined.

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The aldehydes are generally used for tanning agents. A modified method for assessing bound form aldehyde in tanned collagen by means of gas liquid chromatography (GLC) was described. The method consists of hydrolysis of collagen tanned with form aldehyde. conversion of the aldehyde into 2, 4-dinitrophenyl hydrazone (DNPH) and direct determination of DNPH by GLC. By this method, about 98% of formaldehyde bound to collagen can be determined.

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The thiamine destruction by an extract of Laetiporus versisporus is not stimulated by pyridine but that by Boletus rubellus and Lactarius piperatus is remarkably stimulated. Thiamine-decomposed products by these mushrooms were studied by means of gaschromatography. The results obtained were summarized as follows.
1. 4-Methyl-5-β-hydroxyethyl thiazole (Th) was found mainly in the ether soluble part.
2. 2-Methyl-4-amino-5-hydroxymethyl pyrimidine (Pm) was found mainly in the butanol soluble part.
3. In the case of Lactarius piperatus, besides a big peak of Th, some small peaks of unknown substances were found, however, when pyridine was added, only one peak of pyrithiamine was observed.
4. In the case of Boletus rubellus, pyrithiamine did not appear when pyridine was added. Retention time of the main products was 3.2 and 11 minutes (pyrithiamine, 2.7).
5. In the case of Laetiporus versisporus, peaks of other unknown substances were much found than that of Th and Pm.

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N^α-cocoyl-L-arginine ethyl ester DL-2-pyrrolidone-5-carboxylic acid salts (abbreviation: CAE) was prepared by neutralization with pyrrolidone carboxylic acid after condensing fatty acids of coconut oil and L-arginine ethyl ester. Tests for protection of plant pathogens were performed because of wide antimicrobial action of CAE.
As the results of investigation in laboratory, CAE in the medium had shown remarkable antimicrobial effects to anthracnose of banana, rust of broad bean, sheath blight of rice plant, black spot of pear respectively.
Field tests were carried out and growth inhibitory actions to plant pathogens were clearly shown at such concentration as 500 μg/ml to chocolate or red spot of broad bean, 70 μg/ml to black spot of pear and scab of pear, 150 μg/ml to rust of pear, and 500 μg/ml to bacterial leaf spot of cucumber and downy mildew of cucumber. A slightly inhibitory action to powdery mildew of cucumber was also found.

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The essential oil of a wild mint, a strain of Mentha gentilis L., containing (+)-piperitone as a major component was investigated. The variation of the content of the following constituents in the oils harvested in the different seasons (July, August, October and November) was also examined: piperitenone (17.1, 14.5, 1.9 and 0.3%), (+)-piperitone (13.4, 13.2, 22.1 and 22.6%), (+)-pulegone (30.5, 30.0, 3.9 and 1.0%), (-)-menthone (14.5, 17.3, 21.5 and 9.7%), (-)-menthol (5.3, 5.1, 17.6 and 20.3%), (-)-menthyl acetate (2.2, 2.4, 11.8 and 21.8%) and (+)-1, 2-epoxyneomenthyl acetate (1.5, 1.3, 6.9 and 14.2%). The content of piperitone in the plant harvested in October and November was found to accumulate in contrast with piperitenone and pulegone.

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It was found that the addition of nonionic surfactants, having polyoxyethylenic groups, could accelerate the rate of crystallization of hen egg white lysozyme (3, 2, 1, 17) in acidic pH, to widen the conditions of lysozyme crystallization. Crystals of lysozyme hydrochloride were obtained directly from egg white with this effect.
Four types of nonionic surfactants tested, were all effective, and in the same type of the surfactant, the more hydrophilic, the more effective. Although the effect increased in proportion to the concentration of the surfactant added, the effect reached a plateau at above the definite concentration of the surfactant.
A small amount of the surfactant was detected in crystals of lysozyme obtained in the presence of the surfactant.

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