Combined Effect of Tylosin, Furylfuramide and Sorbic Acid

Abstract

The effect of sorbic acid on the antibacterial activity of tylosin and furylfuramide was examined against many strains, mainly identified as Bacillus, isolated from various sources. The growth of tylosin- and furylfuramide- resistant strains, respectively, was inhibited with fairly high frequency by using sorbic acid with those preservatives. The experiments employing the drug-sensitive Bacillus subtilis var. niger indicated that the spore germination was considerably inhibited by sorbic acid as increasing the concentration of sodium chloride in test media and the outgrowth of spores was also synergistically inhibited by the combined use of either tylosin and NaCl or tylosin, sorbic acid, and NaCl. But in the case of furylfuramide the combined effect was not significant.
From these results, it may be concluded that sorbic acid enhanced the antibacterial activity of tylosin on both tylosin-resistant and -sensitive bacilli and its enhancing action was considerably affected by the available moisture content (A_W) of food.