Food Chemical Studies on Soybean Phospholipids

Part IV. Fatty Acid Component of 3-sn-phosphatidylcholine

Abstract

Employing soybean 3-sn-phosphatidylcholine was separated by thin-layer chromatography on silica impregnated with silver nitrate into 4 fractions depending on their degree of unsaturation and these fractions were designated as SBL-1, SBL-2, SBL-3, and SBL-4 according to Rf value. The fatty acid components of the SBL-1 were oleic acid (8.2%) and linoleic acid (46.9%) as unsaturated fatty acids. The fatty acid component of the SBL-2 was linoleic acid (over 93.2%) as unsaturated fatty acid. The fatty acid components of the SBL-3 were linolenic acid (24.0%) and linoleic acid (21.4%) as unsaturated fatty acids. The fatty acid component of the SBL-4 was arachidonic acid (63.8%) as unsaturated fatty acid. On the other hand, the 3-sn-phosphatidylcholine was separated into 9 fractions by adsorption column chromatography on silica impregnated with silver nitrate and these fractions were designated as fraction A, A', B, B', C, C', D, E, F and H. The fatty acid components of these fractions were revealed, while the fraction A corresponded to the SBL-2 and the fraction E corresponded to the SBL-4.