Chemical Studies on Peroxidase of the Lotus (Nelumbo nucifera, Gaertn) Rhizomes

Part I. Characteristics of peroxidase in the Lotus rhizomes and its zone electrophoresis in crude

Abstract

This work was undertaken to investigate the separation and purification of peroxidase in the Lotus rhizomes.
Experiments were carried out on the changes in peroxidase activity under some condition, optimum pH, thermostability and zone electrophoresis for peroxidase in crude in the Lotus rhizomes.
The results obtained showed that peroxidase activity increased during incubation at 30°C, optimum pH was 4.6 and the enzyme was inactivated with the rising of the temperature. Electrophoresis using cellulose acetate membrane and pentaacetyl glucose as the medium gave two bands for the peroxidase in crude.