Studies on the Check of Quality of Japanese Horseradish ("Wasabi") Powder by Gas Chromatography

Part I Application of isothermal temperature column

Abstract

This study was carried out with the purpose to check the quality of "Wasabi" powder by gas chromatography.
(1) Column used for gas chromatography were 10% SE-30/Celite 545; 5% SE-30/Chromosorb W; 2% OV-17/Chromosorb W and 2.5% DEGS/Celite 545. Temperature of column bath was 125°C and flow rate of carrier gas (He) was 12.5ml/min.
(2) The peaks of both allyl and β-phenylethyl isothiocyanate were separated clearly. Ratio of peak area of the latter to that of the former was calcurated by percentage, to be noted as R value.
(3) The R values of horseradish powder were large, whereas those of black mustard powder were small.
(4) The R values of six "Wasabi" powder (c), horseradish powder (a), and black mustard powder (b) were put into next formula.
ax+b(100-x)=100c
where x indicated the rate of horseradish powder in "Wasabi" powder, because (100-x) indicated the rate of black mustard powder. The x values of two samples of six "Wasabi" powders were below fifty percent.
(5) One sample of six "Wasabi" powders showed above ten percent in ash content.