Pectinase Preparation for Making Clarified Orange Juice

Part V. On the accelerating factor for the clarification in Hirotake (Tramates sanguina) cellulase preparation

Abstract

In the previous paper, it was reported that a fraction free from polygalacturonase and pectinesterase activities in a commercial cellulase preparation from Hirotake contained an accelerating factor for the clarification.
In this paper, more detailed investigation was carried out to obtain the following results. (a) An accelerating factor for the clarification existed in an acid and thermo-stable hemicellulase fraction. (b) An accelerating effect was not seen for active parts of hydrocellulase and carboxymethyl cellulase. (c) An accelerating effect for clarification was seen in a xylanase active part of hemicellulase, but its detailed role was not revealed.