1971 Volume 18 Issue 12 Pages 574-582
Purification of the accelerating factor for the clarification in Hirotake cellulase preparation was carried out by some methods such as salting out with ammonium sulfate and gel filtration with DEAE-sephadex A-50, sephadex G-200 and Duolite A-2, and was found that these methods were the most suitable to that purpose.
The obtained accelerating factor by this method contains a xylanase which is smaller in molecular weight and more acidic stable than general xylanase, and also contains a hemicellulase fraction which reacts to a wheat hemicellulose (D) in the acidic medium.
This factor also reacts to the citrus hemicellulose from which made citrus natsudaidai carpel and juice precipitate. Araban in citrus pectin was degraded by this factor in acidic region of reaction medium, but the reaction mechanism was unknown in details in what sorts of enzyme was concerned in the factor.
