Studies on the Green Pigments formed from Chlorogenic Acid and Amino Acid

Part I. Isolation and purification oi green pigments on Sephadex column chromatography

Abstract

The studies on the mechanism of greening resulted from the chemical reaction of chlorogenic acid or its related compounds with amino acids, peptides or proteins had been carried out by URITANI et. al in detail. But the chemical structure and properties have not been elucidated yet. So the authors tried to make clear these problems.
The green solution was prepared from the mixture of chlorogenic acid and L-phenylalanine by two methods of oxidation, polyphenoloxidase in the presence of air and only air. Each green solution prepared was treated by Sephadex G-25 and fractionated into three color bands (brown, green and blue-purple), respectively. Of these, the green and blue-purple fractions were purified and powdered by acetone treatment. In this paper, the method of fractionation and some chemical properties of the powdered pigments are described.