High Boiling Components in Neutral Oil of Haccho Miso

Abstract

Neutral oil in miso was obtained by metanol extraction, ether extraction, acid and alkaki treatments.
The high boiling fraction of neutral oil was separated by steam distillation, and the high boiling fraction was fractionated further into six fractions (peak 37, 38, 39, 40, 51 and 42) by preparative gaschromatography.
Identification of these components was carried out using IR, Mass and NMR spectrometry.
Peak 37, 38, 39, 40 and 41 were identified as methyl ester of palumitic, stearic, oleic, linoleic and linolenic acid respectively, and the investigations on the structure of peak 42 are now in progress.