Nucliec acid Degrading Enzyme of Matsu-take (Tricholoma matsutake)
1972 Volume 19 Issue 6 Pages 249-254
Details
1972 Volume 19 Issue 6 Pages 249-254
Enzyme fractions decomposing nucleic acid and related substances were isolated from the fruiting body of Matsu-take (Tricholoma matsutake), and they were partially purified by chromato- graphy using DEAE-Cellulose column.
Ribonuclease (RNase) from Matsu-take had an optimum temperature of 60°C and a pH range of 7.0-7.5. The ranges of optimum temperature and pH of phosphodiesterase and phosphomono- esterase were 40-45°C and pH 6.0-6.5 and 40-45°C and pH 4.5-4.8, respectively.
Yeast RNA was degraded by RNase fraction of Matsu-take under optimum condition for its activity, and four kinds of 5'-nucleotides, namely 5'-CMP, 5'-UMP, 5'-AMP and 5'-GMP were accumulated abundantly in its digest. The acid range-enzyme activities of fruiting bodies are different from those of Shii-take, and common mushrooms.
